Trypsin ethylenediaminetetraacetic acid edta 0.25

Manufactured by Thermo Fisher Scientific

Sourced in United States, Australia

Trypsin–ethylenediaminetetraacetic acid (EDTA) (0.25%) is a cell dissociation reagent used for the detachment of adherent cells from cell culture surfaces. It contains the proteolytic enzyme trypsin and the chelating agent EDTA, which work together to break down the extracellular matrix and cell-cell junctions, enabling the release of cells from the substrate.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Cell culture vessels, by Corning (1 mentions) 4 6 diamidino 2 phenylindole dapi propidium iodide, by Solarbio (1 mentions) Alexa fluor 488, by Solarbio (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Triton x, by Merck Group (1 mentions) Triton x, by HiMedia (1 mentions) Cholera toxin b subunit ctxb, by Merck Group (1 mentions) Paraformaldehyde (pfa), by HiMedia (1 mentions) Moviol, by Merck Group (1 mentions) Ammonium persulfate, by HiMedia (1 mentions) Rat tail collagen 1, by Thermo Fisher Scientific (1 mentions) Ethidium bromide, by HiMedia (1 mentions) Triton x 100, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Hplc grade acetonitrile, by RCI Labscan (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

Spelling variants of this product

Trypsin-EDTA (7300 citations) Trypsin-EDTA (0.25%) (187 citations) Trypsin-ethylenediaminetetraacetic acid (159 citations) Ethylenediaminetetraacetic acid (103 citations) Trypsin-ethylenediaminetetraacetic acid (EDTA) (89 citations) Ethylenediaminetetraacetic acid (EDTA) (63 citations) Trypsin 0.25% (26 citations) Trypsin-ethylenediaminetetraacetic acid (trypsin-EDTA) (15 citations) Trypsin-0.25% ethylenediaminetetraacetic acid (2 citations) Trypsin-EDTA (0.25% trypsin-EDTA) (2 citations)

5 protocols using trypsin ethylenediaminetetraacetic acid edta 0.25

Synthesis and Characterization of Cytotoxic Agents

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Cet was obtained from Merck KGaA (Darmstadt, Germany). CisPt was obtained from Boyuan Chemical Company (Shandong, China). Succinic anhydride (SA), 1-isocyanatooctane (C8-NCO), acetone, hydrogen peroxide, ether, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), EDCI, and NHS were obtained from Aladdin (Shanghai, China). Unless otherwise specified, all chemicals were acquired from commercial suppliers and utilized without additional purification. The cell culture vessels were obtained from Corning Inc. (Corning, NY, USA). Dulbecco's modified Eagle's medium (DMEM) containing 4.5 g of glucose, Roswell Park Memorial Institute-1640 (RPMI-1640), trypsin-ethylenediamine tetraacetic acid (EDTA) (0.25%), penicillin/streptomycin, and fetal bovine serum were procured from Gibco (Grand Island, NY, USA). 4',6-diamidino-2-phenylindole (DAPI), propidium iodide (PI), Alexa Fluor® 488, and the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) apoptosis assay kit were acquired from Solarbio Science & Technology Co., Ltd. (Beijing, China). More detailed information about materials and method are shown in the Supplementary data.

Yin X., Zhuang Y., Song H., Xu Y., Zhang F., Cui J., Zhao L., Yu Y., Zhang Q., Ye J., Chen Y, & Han Y. (2023). Antibody-platinum (IV) prodrugs conjugates for targeted treatment of cutaneous squamous cell carcinoma. Journal of Pharmaceutical Analysis, 14(3), 389-400.

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Cellular Imaging and Protein Identification

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All oligonucleotides (SI Table 1), moviol, Triton-X, loading dye, Alexa Fluor (phalloidin) 488, moviol, poly l lysine, membrane glycoproteins (CD98 and CD147), transferrin 488, galectin, Hoechost (DAPI), Cholera toxin B-subunit (CTxB), were purchased from sigma Aldrich. Ammonium persulfate, ethidium bromide, triton X, Tetramethylethylenediamine (TEMED), Paraformaldehyde and cell culture dishes for adherent cells (treated surface) were procured from Himedia. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, trypsin-Ethylenediaminetetraacetic acid (EDTA) (0.25%), Collagen1 rat tail were purchased from Gibco. Tris-acetate-EDTA (TAE), Acrylamide/Bisacrylamide sol 30% (29:1 ratio for SDS PAGE) were procured from GeNei. Magnesium chloride, NaCl, KCl, Na 2 HPO 4 , and KH 2 PO 4 were purchased from SRL, India and Santa cruez biotech respectively.

Walia S., Morya V., Gangrade A., Naskar S., Teja A.G., Dalvi S.V., Maiti P.K., Ghoroi C., & Bhatia D. (2021). DESIGNER DNA HYDROGELS TO STIMULATE 3D CELL INVASION BY ENHANCED RECEPTOR EXPRESSION AND MEMBRANE ENDOCYTOSIS.

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Synthesis and Characterization of Nanoparticles

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Cetyltetramethylammonum bromide (CTAB), tetraethyl orthosilicate (TEOS), FBZ, phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO), tetraethyl orthosilicate (TEOS), D-α-tocopherol polyethylene glycol succinate (TPGS), carbonyldiimidazole (CDI), (3-Aminopropyl)triethoxysilane (APTES), sodium hydroxide (NaOH), 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI), phalloidin-FITC (fluorescein isothiocyanate), paraformaldehyde, bovine serum albumin (BSA), and triton X-100 were purchased from Merck (Castle Hill, NSW, Australia). Roswell Park Memorial Institute (RPMI-1640), trypsin–ethylenediaminetetraacetic acid (EDTA) (0.25%), fetal bovine serum (FBS), acetone, and formic acid (FA) were from Thermo Fisher Scientific (Scoresby, VIC, Australia). MTT and 2′-7′dichlorofluorescin diacetate (DCFH-DA) were purchased from Abcam (Melbourne, VIC, Australia) and PromoKine (Promocell GmbH, Germany), respectively. Cyanine5 NHS ester (Cy-5) was purchased from Tocris Bioscience, Australia. HPLC-grade acetonitrile was from RCI Labscan (Bangkok, Thailand). Deionized double-distilled water (Milli-Q water) was used in all experiments. PC-3 cells were kindly supplied by Dr. Jennifer Gunter (Queensland University of Technology, Brisbane, QLD, Australia).

Esfahani M.K., Alavi S.E., Cabot P.J., Islam N, & Izake E.L. (2021). PEGylated Mesoporous Silica Nanoparticles (MCM-41): A Promising Carrier for the Targeted Delivery of Fenbendazole into Prostrate Cancer Cells. Pharmaceutics, 13(10), 1605.

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Maintaining and Cryopreserving NIH3T3 and HEK293T Cell Lines

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NIH3T3 (American Type Culture Collection (ATCC), catalog no. CRL-1658) and HEK293T cells (ATCC, catalog no. CRL-3216) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), 2 mM l-glutamine and 100 U ml⁻¹ of penicillin–streptomycin (Thermo Fisher Scientific), and cultured at 37 °C and 5% CO₂. For subculturing, medium was aspirated, cells were washed with PBS and detached with trypsin–ethylenediaminetetraacetic acid (EDTA) 0.25% (Thermo Fisher Scientific). Detachment reactions were stopped with culture medium and cells were seeded at the desired densities. Cell stocks were prepared by resuspending cell aliquots in FBS with 10% dimethyl sulfoxide and freezing them slowly at −80 °C. Frozen aliquots were then moved to liquid nitrogen for long-term storage. All cell lines were regularly tested for Mycoplasma contamination.

Kaye R.E., Fruman D.A., Bierer B.E., Albers M.W., Zydowsky L.D., Ho S.I., Jin Y.J., Castells M.C., Schreiber S.L, & Walsh C.T. (1992). Effects of cyclosporin A and FK506 on Fc epsilon receptor type I-initiated increases in cytokine mRNA in mouse bone marrow-derived progenitor mast cells: resistance to FK506 is associated with a deficiency in FK506-binding protein FKBP12.. Proceedings of the National Academy of Sciences of the United States of America, 89(18), 8542-8546.

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2D Expansion of HepaRG Cells

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HepaRG from Biopredic (Rennes, France) cells were expanded in two-dimensional (2D) monolayers following the indications reported by the supplier. Cells were passaged every 2 weeks until passage 18, with proliferation culture medium William's E (WE, with sodium bicarbonate, without L-glutamine and phenol red, Sigma-Aldrich, with added Biopredic 710 proliferation media) replenishment thrice a week.
The cultures were maintained in a humidified environment at 37°C, 5 % CO 2 . After that, cells were detached by trypsin-ethylenediaminetetraacetic acid (EDTA) 0.25% (ThermoFisher scientific) from the culture flasks and used for the cell encapsulation process (explained in paragraph 2.2).

Pasqua M., Pereira U., Messina A., de Lartigue C., Vigneron P., Dubart-Kupperschmitt A, & Legallais C. (2020). HepaRG Self-Assembled Spheroids in Alginate Beads Meet the Clinical Needs for Bioartificial Liver. Tissue engineering. Part A, 26(11-12).

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