Ag 014699

Manufactured by Selleck Chemicals

Sourced in China

AG-014699 is a laboratory reagent used for research purposes. It is a chemical compound that can be utilized in various experimental procedures. The core function of AG-014699 is to serve as a research tool in scientific investigations, without further interpretation or extrapolation on its intended use.

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Lab products found in correlation

Bmn673, by Selleck Chemicals (2 mentions) Azd2281, by Selleck Chemicals (2 mentions) Rucaparib, by Selleck Chemicals (1 mentions) Talazoparib, by Selleck Chemicals (1 mentions) Pdd00017273, by Bio-Techne (1 mentions) N7004, by Merck Group (1 mentions) C7698, by Merck Group (1 mentions) M7449, by Merck Group (1 mentions) Olaparib, by Selleck Chemicals (1 mentions) Nitd008, by Merck Group (1 mentions) Mg132 m8699, by Merck Group (1 mentions) Hek293t, by American Type Culture Collection (1 mentions) Bhk 21, by American Type Culture Collection (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Axiovision 4, by Zeiss (1 mentions) Nec 1, by Merck Group (1 mentions) Z vad, by Merck Group (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Olaparib, by LC Laboratories (1 mentions)

5 protocols using ag 014699

Characterization of Breast Cancer Cell Lines

Cited 3 times

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Human breast cancer cell lines were obtained from the American Type Culture Collection (Manassas, VA) and from the cell repository of the Hamon Center for Therapeutic Oncology Research at UT Southwestern. Cell lines were MAP tested and fingerprinted (20 (link)). MDA-MB-231 (231) cells knocked down for K-H or PARP1 expression were generated (3 (link),20 (link),21 (link)) using specific lentiviral shRNAs and maintained in RPMI media supplemented with 5–10% fetal bovine serum (FBS) at 37 ºC in a humidified CO₂ (5%) incubator. AG014361, AG014699 (Rucaparib) and RO3066 (CDK1 inhibitor) were purchased from Selleck Chemicals (Houston, TX) at the highest purity. All cell lines were routinely checked and found free of mycoplasm infection. Western blots, transfections, immunofluorescence (IF), co-immunoprecipitations, TUNEL, and colony forming assays (CFA) were performed using standard protocols. Specific experimental details are included in the Supplemental Information.

Motea E.A., Fattah F.J., Xiao L., Girard L., Rommel A., Morales J.C., Patidar P.L., Zhou Y., Porter A.C., Xie Y., Minna J.D, & Boothman D.A. (2018). Kub5-HeraRPRD1B deficiency promotes “BRCAness” and vulnerability to PARP inhibition in BRCA-proficient Breast Cancers. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(24), 6459-6470.

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Evaluating PARP Inhibitors on Cellular Responses

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The following compounds were used at the indicated final concentrations: PJ-34 (10 μM, ALX-270-289-M001, Enzo Life Science), Olaparib (1-10 μM, AZD-2281, Selleckchem), Rucaparib (1-8 μM, AG-014699, Selleckchem), Talazoparib (6.25-50 nM, BMN673, Selleckchem), β-Nicotinamide adenine dinucleotide hydrate (20-200 μM, N7004, Sigma-Aldrich), hydrogen peroxide (0.02-0.5 mM, H3420, Sigma-Aldrich), Methyl methanesulfunate, MMS (0.01%, 129925, Sigma-Aldrich), PARG inhibitor (10 μM, PDD00017273, Tocris), FK866 (1 μM, F8557, Sigma-Aldrich), MG132 (10 μM, M7449, Sigma-Aldrich), cycloheximide (50 μM, C7698, Sigma-Aldrich).

Gatti M., Imhof R., Huang Q., Baudis M, & Altmeyer M. (2020). The Ubiquitin Ligase TRIP12 Limits PARP1 Trapping and Constrains PARP Inhibitor Efficiency. Cell Reports, 32(5), 107985.

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Cytotoxicity Assay of HepG2 Cells

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The HepG2 cells were provided by the Institute of Modern Physics, Chinese Academy of Science (Lanzhou, China). DMEM was purchased from Hyclone; GE Healthcare Life Sciences (Logan, UT, USA); fetal bovine serum (FBS) was purchased from Sijiqing Company (Hangzhou, China). MTT was purchased from Amresco, LLC (Solon, OH, USA), BSI-201 was purchased from Sigma; Merck KGaA (Darmstadt, Germany). AZD2281 and AG014699 were purchased from Selleck Chemicals (Shanghai, China). The primary antibodies (GAPDH, Caspase 3, Caspase 8, Bcl-2, Bax, PTEN, TIMP 3 and MMP3) were purchased from Affinity Biologicals Inc. (Ancaster, ON, Canada). The secondary antibody was purchased from Beyotime Institute of Biotechnology (Haimen, China).

Mao X., Du S., Yang Z., Zhang L., Peng X., Jiang N, & Zhou H. (2017). Inhibitors of PARP-1 exert inhibitory effects on the biological characteristics of hepatocellular carcinoma cells in vitro. Molecular Medicine Reports, 16(1), 208-214.

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ZIKV Infection Dynamics in Cell Lines

Cited 1 time

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ZIKV strain GZ01/2016 (GenBank accession number KU820898) was used at a multiplicity of infection (MOI) of 0.1 in this study, except where indicated otherwise (47 (link)). MEF cells were isolated from GD14.5 Balb/c mice. The IFNAR1^−/− A549 cell line was generated as described (20 (link)). A549, BHK-21, Vero, HeLa, and HEK293T cells were purchased from American Type Culture Collection and cultured in Dulbecco’s modified Eagle’s medium (DMEM) (37°C, 5% CO₂) supplemented with 10% fetal bovine serum (FBS; 100 U/ml), penicillin, and streptomycin (50 μg/ml). INO-1001 (S1132) and AG-014699 (S1098) were purchased from Selleck. MG132 (M8699) was purchased from Sigma-Aldrich, and NITD008, an adenosine nucleoside analog inhibitor that inhibits the RdRp (RNA-dependent RNA polymerase) activity of Flavivirus (48 (link)), was a gift from P.-Y. Shi (Novartis Institute for Infectious Diseases).

Li L., Zhao H., Liu P., Li C., Quanquin N., Ji X., Sun N., Du P., Qin C.F., Lu N, & Cheng G. (2018). PARP12 suppresses Zika virus infection through PARP-dependent degradation of NS1 and NS3 viral proteins. Science signaling, 11(535), eaas9332.

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Neuronal Cell Death Pathways

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Two to three month old human cortical neurons were treated with NMDA or oxygen-glucose deprivation at various indicated time duration and doses. Percent of cell death was determined by the staining with 5 µM Hoechst 33342 and 2 µM propidium iodide (PI) (Invitrogen, Carlsbas, CA). Images were taken and counted by Zeiss microscope equipped with automated computer assisted software (Axiovision 4.6, Zeiss). 20 µM Z-VAD (Sigma, V116), 20 µM NEC-1 (Sigma, N9037), 500 µM 3-MA (Calbiochem, 189490), 20 µM NPLA (Tocris, 1200), 30 µM DPQ (Enzo, ALX-270-21-M005), 500 nM AG-014699 (Selleckchem, S1098) 10 µM ABT888 (Active biochem, A-1003), 2 µM Olaparib (LC laboratories, O-9201) and 20 nM BMN673 (Selleckchem, S7048) were applied to evaluate the effect of different antagonists to known cell death signals.

Xu J.C., Fan J., Wang X., Eacker S.M., Kam T.I., Chen L., Yin X., Zhu J., Chi Z., Jiang H., Chen R., Dawson T.M, & Dawson V.L. (2016). Cultured networks of excitatory projection neurons and inhibitory interneurons for studying human cortical neurotoxicity. Science translational medicine, 8(333), 333ra48.

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