1 3 dimethylamino propyl 3 ethylcarbodiimide methiodide

Manufactured by Merck Group

Sourced in United States

1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide methiodide is a water-soluble carbodiimide compound used in biochemical and organic synthesis applications. It functions as a coupling agent to facilitate the formation of amide bonds between carboxylic acids and primary amines.

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2 protocols using 1 3 dimethylamino propyl 3 ethylcarbodiimide methiodide

Synthesis and Characterization of Gold Nanoparticles

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All chemicals were used as received without further purification. The following chemicals were received from Sigma Aldrich, Inc. (St. Louis, MO, USA): gold(III) chloride trihydrate (HAuCl₄⋅3H₂O, ≥99.9%, trace metals basis), L-glutathione reduced (GSH, ≥98.0%), 9-fluorenylmethoxycarbonyl chloride (Fmoc chloride, 97%), tetrahydrofuran (THF, ≥99.9%, anhydrous), dopamine hydrochloride, potassium nitrate (KNO₃, 99.999%), phosphate-buffered saline (PBS) buffer, 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide methiodide (EDC), N-hydroxy-succinimide (NHS, 98%), triethylamine (TEA, ≥99%), gold standard for ICP (1000 mg/L Au in HCl), and deuterium oxide (D₂O, 99.9 atom % D). Sodium bicarbonate (NaHCO₃, ≥99.0%) was obtained from Daejung Inc. (Gyeonggi, Korea). Hydrochloric acid (HCl, 35.0~37.0%) and nitric acid (HNO₃, 60.0%) were purchased from Samchun Chemical Co., Ltd. (Seoul, Korea). Amicon^® Ultra-4 centrifugal ultrafiltration filters (3 kDa MWCO) were purchased from Merck Millipore Corp. (Danvers, MA, USA). Deionized water (DI water, 18 MΩ⋅cm) was used in the preparation of aqueous solutions (Aquapuri541, YOUNG IN Chromass, Gyeonggi, Korea).

Kim J.H, & Kim J. (2020). Post-Synthesis Modification of Photoluminescent and Electrochemiluminescent Au Nanoclusters with Dopamine. Nanomaterials, 11(1), 46.

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Monocrystalline Si Cantilever Arrays

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Octosensis microcantilever arrays (chips
with eight monocrystalline Si cantilevers, 500 μm in length)
were obtained from Micromotive GmbH (Mainz, Germany). The following
chemicals were purchased from Sigma-Aldrich and used as received without
further purification: isopropanol, (3-glycidyloxypropyl)trimethoxysilane
(98%); toluene (99.8%); N^α,N^α-bis(carboxymethyl)-l-lysine
hydrate (NTA–NH₂) (97%); 1-[(3-dimethylamino)propyl]-3-ethylcarbodiimide
methiodide (EDC); N-hydroxysuccinimide (NHS), 2-(N-morpholino)ethanesulfonic acid (MES); 2-amino-2-(hydroxymethyl)-1,3-propanediol
(Tris base); carbonate/bicarbonate buffer; and phosphate buffered
saline (PBS, pH 7.4). Sodium chloride was purchased from Panreac (Spain).
BSA (fraction V) and LYS were purchased from BioLabs (England) and
Fluka, respectively. The Omp2a protein was expressed, purified, and
refolded using a previously reported procedure.^{22 (link),23 (link)}

Lopes-Rodrigues M., Puiggalí-Jou A., Martí-Balleste D., del Valle L.J., Michaux C., Perpète E.A, & Alemán C. (2018). Thermomechanical Response of a Representative Porin for Biomimetics. ACS Omega, 3(7), 7856-7867.

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