Dna ladder

Manufactured by Takara Bio

Sourced in China

A DNA ladder is a molecular weight standard used in gel electrophoresis to determine the size of DNA fragments. It contains a mixture of DNA fragments of known lengths, which can be used to estimate the size of unknown DNA samples.

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100 bp DNA ladder (16 citations) DNA ladder marker (9 citations) 50-bp DNA ladder (6 citations) 20 bp DNA Ladder (Dye Plus) (6 citations) 20 bp DNA ladder (4 citations) DL2000 DNA ladder (3 citations) 1 kb DNA ladder (3 citations) 100 bp DNA Ladder (3422A) (2 citations) 100 bp plus DNA ladder marker (2 citations) 2 kb DNA Ladder (2 citations)

4 protocols using dna ladder

Dumbbell Probe Nucleic Acid Analysis

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The dumbbell probe and the related nucleic acids
were purchased and purified from Shanghai Sango Biotechnologies Co.,
Ltd. (Shanghai, China). The obtained nucleic acids from Shanghai Sango
Biotechnologies Co., Ltd. (Shanghai, China) were then rehydrated into
a concentration at 10 μM. The experimental enzymes, including
Phi29 polymerase, Nb.BbvCI enzymes were purchased
from Thermo Fisher Scientific (Waltham, MA). dNTP mix, bovine serum
albumin (BSA), and DNA ladder were brought from TaKaRa Biotech Company
(Dalian, China). Twelver percent PAGE gel related reagents were all
brought from Bio-Rad (Shanghai) Life Science Research and Development
Co., Ltd. (Shanghai, China). Clinical samples were from Zhuji Affiliated
Hospital of Shaoxing University.

Xu S, & Lou Z. (2021). Ultrasensitive Detection of Nasopharyngeal Carcinoma-Related MiRNA through Garland Rolling Circle Amplification Integrated Catalytic Hairpin Assembly. ACS Omega, 6(9), 6460-6465.

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Peptide Synthesis and Bacteriological Assays

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All peptides with purity >95% were synthesized by China Peptides Co., Ltd. (Shanghai, China). The test strains E. coli ATCC 25922 and S. aureus ATCC 25923 were kindly donated by Prof. Yizhen Wang (Zhejiang University, Hangzhou, China). E. coli DH5α, E. coli (DE3) pLysS competent cells, restriction enzymes KpnI, XhoI, and enterokinase, T4 DNA ligase, DNA ladder and pre-stained protein marker were purchased from Takara (Dalian, China). The pET32a(+) plasmid were purchased from Invitrogen (Beijing, China). Dulbecco’s modified Eagle’s medium (DMEM), penicillin/streptomycin, 0.25% trypsin-ethylenediaminetetraacetic acid (EDTA), and fetal bovine serum (FBS) were purchased from Life Technologies (Grand Island, NY, USA). Other reagents were obtained either from Sangon Chemical Reagent (Shanghai, China) or Sigma (St. Louis, MO, USA). PCR primers were synthesized by Shanghai Sangon Biological Engineering Technology and Services Co., Ltd. (Shanghai, China).

Xu C., Guo Y., Qiao X., Shang X., Niu W, & Jin M. (2017). Design, Recombinant Fusion Expression and Biological Evaluation of Vasoactive Intestinal Peptide Analogue as Novel Antimicrobial Agent. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(11), 1963.

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Molecular Cloning and Expression of PTEN

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The expression vector pGEX-6p-1 was originally obtained from Amersham (Shanghai China). The pGEM-T-easy vector was purchased from Promega (Shanghai China). The BL21 recipient bacterium strain was purchased from Beijing DingGuo ChangSheng Biotechnology (Peking China). A plasmid mini preparation kit was purchased from Omega Biotek (Guangzhou China). DNA polymerase, Restriction endonucleases, T4 DNA ligase, IPTG, protein molecular weight markers, DNA ladder and DNA Gel Extraction Kit were obtained from TaKaRa (Dalian China). The PCR primers were synthesized by Sangon Biotech (Shanghai China). The ProteinPure GST Resins were purchased from TransGen Biotech (Peking China). The Coomassie Brilliant Blue R250 and BSA were purchased from Sigma (Shanghai China) and the PTEN primary antibody was obtained from Santa Cruz (Shanghai China). The ECL Kit was purchased from Boster (Wuhan, China).

Hu Y., An Y., Fang N., Li Y., Jin H., Nazarali A, & Ji S. (2015). The Optimization of Soluble PTEN Expression in Escherichia coli. The Open Biochemistry Journal, 9, 42-48.

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Exonuclease III-Mediated DNA Sequencing

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DNA sequences were synthesized and purified by Sangon Biotechnology Co., Ltd. (Shanghai, China). Exonuclease III (Exo III), NEBuffer 1, and loading buffer were purchased from New England Biolabs (Singapore). DNA ladder was purchased from Takara Biotechnology Co. Ltd. (Dalian, China). Tris–acetate–ethylenediaminetetraacetic acid buffer (50 ×) was purchased from Axil Scientific Pte Ltd. Acrylamide/bis mixed solution [30% (w/v] (29:1) was purchased from Nacalai Tesque, Inc. Ammonium persulfate and tetramethylethylenediamine were purchased from Biorad. All these chemicals were used without further purification. The Milli-Q water was obtained through a Milli-Q system (Millipore) and was used in all the experiments.

Hu Y., Tan C., Lin X., Lai Z., Zhang X., Lu Q., Feng N., Yang D, & Weng L. (2019). Exonuclease III-Regulated Target Cyclic Amplification-Based Single Nucleotide Polymorphism Detection Using Ultrathin Ternary Chalcogenide Nanosheets. Frontiers in Chemistry, 7, 844.

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