Copper(II) sulphate pentahydrate, citric acid monohydrate, anhydrous sodium carbonate, potassium hexacyanoferrate(II) trihydrate, zinc acetate, calcium carbonate, potassium iodide, sulphuric acid, potassium thiocyanate, starch, phenolphthalein, sodium hydroxide, sodium thiosulfate and sodium chloride were purchased from Kemika (Zagreb, Croatia). Myrtenol standard was obtained from Sigma Aldrich (St. Lois, MO, USA) and C7-C30 saturated alkanes standard from Merck (Darmstadt, Germany). Helium for gas chromatography was obtained from Messer, Austria.
Sodium hydroxide (naoh)
Manufactured by Kemika
Sourced in Croatia
Sodium hydroxide is a highly alkaline chemical compound commonly used in laboratory settings. It is a white, crystalline solid with the chemical formula NaOH. Sodium hydroxide is a strong base that is widely used for various chemical processes, including pH adjustment, neutralization, and cleaning.
Automatically generated - may contain errors
Lab products found in correlation
Sodium carbonate anhydrous, by Kemika (4 mentions)
Sodium chloride (nacl), by Kemika (4 mentions)
Catechin, by Merck Group (4 mentions)
Sodium nitrite, by Merck Group (4 mentions)
Potassium chloride (kcl), by Kemika (3 mentions)
Folin ciocalteu reagent, by Kemika (3 mentions)
Glacial acetic acid, by Merck Group (3 mentions)
Apigenin, by Merck Group (3 mentions)
Aluminium chloride, by Merck Group (3 mentions)
Hydroxytyrosol, by Merck Group (3 mentions)
Pinoresinol, by Merck Group (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Aluminum chloride, by Merck Group (3 mentions)
Hydrochloric acid (hcl), by Kemika (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Lidocaine, by Merck Group (2 mentions)
Penicillin, by Lonza (2 mentions)
Streptomycin, by Lonza (2 mentions)
Gallic acid, by Merck Group (2 mentions)
P coumaric acid, by Merck Group (2 mentions)
16 protocols using sodium hydroxide (naoh)
1
Comprehensive Chemical Reagent Procurement
2
Preparation of Pharmaceutical Standards
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Native human AAG (lot no. SLBJ6840V), carvedilol, (+)-cis-diltiazem hydrochloride, dipyridamole, vinblastine sulfate, (±)-propranolol hydrochloride, imipramine hydrochloride, lidocaine, and DMSO were purchased from Sigma-Aldrich (St. Louis, MO, USA). All standards had a specified purity of ≥97% and have been used without further purification. Sodium phosphate dihydrate and sodium hydroxide were purchased from Kemika (Zagreb, Croatia). Tween® 20 was purchased from Fischer Scientific (Pittsburgh, PA, USA). Guanidine hydrochloride was purchased from AppliChem GmbH (Darmstadt, Germany). Ultrapure water was obtained using SG Ultra Clear UV Plus (SG, Burladingen, Germany). All other chemicals used in the study were of analytical grade or higher purity.
3
Phytochemical Analysis of Botanical Extracts
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All chemicals and reagents used in the experiments were of per analysis and HPLC grade. Folin–Ciocalteu reagent, potassium chloride, sodium hydrogencarbonate, potassium hydrogenphosphate, magnesium chloride hexahydrate, sodium chloride, ammonium carbonate, sodium hydroxide, and petroleter were supplied from Kemika d.d. (Zagreb, Croatia). Anhydrous sodium carbonate and sodium nitrite were acquired from Gram-Mol d.o.o. (Zagreb, Croatia). Sodium molybdate (>98%) was supplied from abcr Gmbh (Karlsruhe, Germany). Methanol, acetonitrile, concentrated hydrochloric acid (37%, v/v), sodium hydroxide, and formic acid were purchased from Carlo Erba Reagents (Val de Reuil, France). Secondary analytical standards, i.e., echinacoside (>98%) and verbascoside (>98%), were obtained from Biosynth s.r.o. (Bratislava, Slovakia), whereas caffeine was supplied from Fluka (Taufkirschen, Germany). Theobromine (min. 99%), human salivary α–amylase XII-A (Enzyme Commission number: 3.2.1.1.; 1149 U mg−1 protein), pepsin from porcine gastric mucosa (474 U mg−1 protein), pancreatin from porcine pancreas (4×USP), and bile bovine salts (unfractionated) were provided from Sigma Aldrich (St. Louis, MO, USA).
4
Cytotoxicity Assay Protocol
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Acetonitrile, ethyl acetate, formic acid and methanol were purchased from Kefo (Sisak, Croatia); Czapek Yeast Agar (CYA) was purchased from Oxoid (Hampshire, UK). Medium RPMI 1640, fetal bovine serum (FBS), phosphate buffered saline (PBS; without Ca2+ and Mg2+), trypsin-EDTA, antibiotics penicillin and streptomycin were from Lonza (Basel, Switzerland). The MTT reagent [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], acridine orange, ethidium bromide, normal melting point agarose (NMP), low melting point agarose (LMP), phorbol 12-myristate 13-acetate (PMA), Triton X-100 and Tris buffer were purchased from Sigma-Aldrich (Deisenhofen, Germany) and molecular biology grade dimethyl sulfoxide (DMSO), used for dissolving the extracts and for the control treatment, was purchased from Sigma-Aldrich (Deisenhofen, Germany); technical grade DMSO, used for dissolving the formazane, ethanol, sodium chloride, disodium-EDTA and sodium hydroxide were from Kemika (Zagreb, Croatia).
5
Determination of beta-blocker standards
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Standards of (±)-atenolol, (±)-acebutolol, (±)-labetalol and (±)-bisoprolol were purchased from Sigma-Aldrich (St. Louis, MO, USA) and (±)-metoprolol tartrate was purchased from Tokyo Chemical Industry (Tokyo, Japan). All standards were of high-purity grade (>98 %). High-purity grade (99 %) triethylamine (TEA) was purchased from Tokyo Chemical Industry, and glacial acetic acid (HAc) from Alkaloid AD Skopje (Skopje, North Macedonia). HPLC-purity grade methanol (MeOH) was purchased from J. T. Baker (Gliwice, Poland). Sodium hydroxide was of p.a. grade and purchased from Kemika (Zagreb, Croatia). Ultrapure water was obtained from a Milli-Q Advantage A10 purification system (Merck, Kenilworth, NJ, USA).
6
Purification and Characterization of Apo-Transferrin
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Native human apo-transferrin (Biorbyt, UK, cat. no. orb80927), sodium acetate trihydrate (Kemika, Croatia), calcium chloride (Lach-Ner, Croatia), sodium chloride (Kemika, Croatia), neuraminidase (GlycoCleave® Neuraminidase Kit, GALAB Technologies, Germany, cat. no. 132011), pISep Buffer Kit (CryoBioPhysica, USA, cat. no. 20055), hydrogen chloride (Carlo Erba Reagents, Italy, 37 % solution), sodium hydroxide (Kemika, Croatia, pellets 2–5 mm), sodium phosphate (Kemika, Croatia), guanidine hydrochloride (PanReac AppliChem, USA), MES (2-(N-morpholino)ethanesulfonic acid, Sigma Aldrich, USA) and potassium chloride (Alkaloid, North Macedonia) were used without further purification. Water used for experiments was double distilled in an all-glass apparatus. All experiments except the enzymatic desialylation were performed at room temperature.
7
Identification and Quantification of Bioactive Compounds
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Syringaldehyde, deuterated chloroform (D, 99.8%), sodium molybdate dihydrate, hydroxytyrosol, apigenin, gallic acid, catechin, and pinoresinol were purchased from Sigma-Aldrich (Steinheim, Germany). Glacial acetic acid, acetonitrile, cyclohexane, aluminium chloride, sodium carbonate anhydrous, sodium nitrite, and methanol (HPLC grade) were obtained from Merck (Darmstadt, Germany). Folin–Ciocalteu’s reagent, tyrosol, and cinnamic acid were bought from Fluka Chemie (Buchs, Switzerland), while oleacein and oleocanthal were obtained from PhytoLab GmbH (Vestenbergsgreuth, Germany) and dacarbazine from Selleck Chemicals (München, Germany). Sodium hydroxide and n-hexane were purchased from Kemika (Zagreb, Croatia). Components for cell culture maintenance, including RPMI 1640, FBS, trypsin-EDTA, phosphate-buffered saline (PBS; Ca2+ and Mg2+ free), penicillin, and streptomycin, were from Lonza (Basel, Switzerland) and the CellTiter 96®AQueous One Solution Cell Proliferation Assay was from Promega (Madison, WI, USA).
8
Antioxidant Compound Quantification Protocol
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Methanol (high-performance liquid chromatography, HPLC grade), 2,2-diphenyl-1-picrylhydrazyl (DPPH), catechin, benzoic acid, hydroxytyrosol, oleuropein, homovanillyl alcohol, p-coumaric acid, pinoresinol and apigenin were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Tyrosol, 3,4-dihydroxybenzoic acid, gallic acid, p-hydroxybenzoic acid, syringic acid, vanillic acid, cinnamic acid, ferulic acid, o-coumaric acid and Folin-Ciocalteu’s reagent were obtained from Fluka Chemie GmbH (Buchs, Switzerland). Formic acid (99+%) and vanillin were purchased from Acros Organics (Morris Plains, NJ, USA). Sodium hydroxide and sodium carbonate anhydrous were purchased from Kemika (Zagreb, Croatia) while sodium molibdate dehydrate, sodium nitrite, glacial acetic acid and aluminum chloride were obtained from Merck (Darmstadt, Germany).
9
Quantification of Phenolic Compounds
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MEthanol and acetonitrile (HPLC gradient grade) were supplied from J. T. Baker ( Germany). Formic acid (LC-MS grade) was purchased from Merck (Germany). Ultra-pure water obtained by the WaterPro water system Labconco (USA) with a resistivity of 18.2 MΩ cm (25 °C) was used in all experiments. Sodium molybdate dihydrate, sodium nitrite, and aluminum chloride were purchased from Merck. Ethanol, dimethyl sulfoxide (DMSO), and oleuropein standard were obtained from Sigma-Aldrich Chemie GmbH (Germany). Folin-Ciocalteu reagent (FC) was purchased from Fluka Chemie GmbH (Switzerland) and sodium carbonate anhydrous and sodium hydroxide were from Kemika (Croatia).
10
Synthesis of Silver Nanoparticles via Hydrothermal Reduction
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All reagents were of analytical grade and solutions were prepared using ultrapure water (Millipore MilliQ system, TKA Germany). Silver nitrate (AgNO3) and N-methyl-2-pyrrolidone (NMP, 99.9 wt%) were obtained from VWR Chemicals (Leuven, Belgium). Poly(acrylic acid) (PAA, Mw = 1800), hydrazine hydrate (N2H4; 50–60 wt%), ethylene glycol (EG; anhydrous, 99.8 wt%) and 3-morpholynopropylamine (MPA) were purchased from Sigma-Aldrich (Burlington, MA, USA). Terpineol (TpOH; mixed isomers, 98 wt%) was purchased from Alfa Aesar (Kandel, Germany). Ethanol (EtOH, 96 wt%), isopropyl alcohol (IPA; anhydrous, 99.5 wt%) and citric acid were obtained from Gram-Mol (Zagreb, Croatia). N,N′-diisopropylcarbodiimide (DCI) was bought from Merck (Darmstadt, Germany) and sodium hydroxide from Kemika (Zagreb, Croatia).
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