Male 6–8 week old NOD/ShiLtJ (NOD) mice were procured from Jackson Laboratory (Bar Harbor, ME). Animal procedures and experiments were all performed according to the protocol approved by the Institutional Animal Care and Use Committee (IACUC) at Northwestern University. The university has an assurance on file (A3283-01) at the Office of Laboratory Animal Welfare. Protocol reviews are conducted according to the regulations set out by the United States Public Health Service (USPHS) and applicable laws. IACUC composition meets all requirements of policies from the USPHS and Animal Welfare Act Regulations. All procedures and experiments performed here adhere to these regulations. Experimental mice were randomized between cages at experimental start point. Blinding was performed for all experiments and subsequent analyses. Control animals were uninfected/untreated naïve mice on which matching behavioral testing and post-experiment analyses were performed.
Nod shiltj nod mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
NOD/ShiLtJ (NOD) mice are a well-established mouse model that is widely used in research. These mice are characterized by the spontaneous development of autoimmune diabetes, making them a valuable tool for studying the pathogenesis and potential treatments for type 1 diabetes.
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Lab products found in correlation
Cellrox, by Thermo Fisher Scientific (1 mentions)
Female cd 1 mice, by Charles River Laboratories (1 mentions)
Apo one homogeneous caspase 3 7 assay, by Promega (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
C3h hej c3h, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Anti hsp90, by Enzo Life Sciences (1 mentions)
Image studio software, by LI COR (1 mentions)
Freestyle freedom lite blood glucose monitoring system, by Abbott (1 mentions)
Glucometer, by Roche (1 mentions)
11 protocols using nod shiltj nod mice
1
NOD Mouse Randomized Behavioral Study
2
Cytokine and ML351 Effects on β-Cell Function
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Mouse βTC3 β-cells were cultured and maintained as previously described (26 (link)). Mouse islets were isolated from collagenase-perfused pancreata as previously described (27 (link)), and human islets were obtained from the Integrated Islet Distribution Program. Cell cultures were treated with a cytokine cocktail containing 5 ng/mL interleukin-1β, 10 ng/mL tumor necrosis factor-α, and 100 ng/mL interferon-γ and/or with ML351 for 24 h. Caspase-3 activation assay was performed by using the Apo-ONE Homogeneous Caspase-3/7 Assay (Promega). Static glucose-stimulated insulin secretion (GSIS) assays and immunostaining for reactive oxygen species (ROS) with CellROX (Invitrogen) were performed as previously described (11 (link)).
Male C57BL/6J mice and female NOD/ShiLTJ (NOD) mice were purchased from The Jackson Laboratory. Female CD1 mice were purchased from Charles River Laboratories. Mice were maintained at the Indiana University vivarium according to protocols approved by the institutional animal care and use committee. Mice were injected intraperitoneally (IP) daily with ML351 dissolved in 80:17:3 PBS:cremophor:ethanol or vehicle alone. Glucose measurements and IP glucose tolerance tests (GTTs) were performed as described (28 (link)). Mice were euthanized, serum/pancreata harvested, and insulin measured as previously described (28 (link)).
Male C57BL/6J mice and female NOD/ShiLTJ (NOD) mice were purchased from The Jackson Laboratory. Female CD1 mice were purchased from Charles River Laboratories. Mice were maintained at the Indiana University vivarium according to protocols approved by the institutional animal care and use committee. Mice were injected intraperitoneally (IP) daily with ML351 dissolved in 80:17:3 PBS:cremophor:ethanol or vehicle alone. Glucose measurements and IP glucose tolerance tests (GTTs) were performed as described (28 (link)). Mice were euthanized, serum/pancreata harvested, and insulin measured as previously described (28 (link)).
3
Mouse Strain Comparison for SLE Research
4
NOD Mouse Islet Isolation and Analysis
5
NOD/ShiLtJ Mice Breeding Protocol
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NOD/ShiLtJ (NOD) mice were procured from The Jackson Laboratory and maintained at the Medical College of Wisconsin (MCW). All mice were bred and maintained under the guidelines of the MCW Institutional Animal Care and Use Committee (IACUC).
6
Generation and Maintenance of NOD Mice
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NOD/ShiLtJ (NOD) mice were originally obtained from The Jackson Laboratory. All mice were bred, maintained, and used in experiments in our pathogen-free animal facility in accordance with the guidelines of the Division of Comparative Medicine at Washington University School of Medicine (Association for Assessment and Accreditation of Laboratory Animal Care accreditation no. A3381-01). NOD.IAPP−/− mice were previously bred in the Haskins mouse colony by backcrossing C57BL6.IAPP−/− mice (20 (link)) onto the NOD background (21 (link)).
7
Coxsackievirus B4 Induces Diabetes in NOD Mice
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Eight-week-old female NOD/ShiLtJ (NOD) mice were purchased from the Jackson Laboratories (001976, The Jackson Laboratories, Bar Harbor, ME, USA) and used for this study, due to their higher incidence of T1D development as they age (50% of females compared to 30% of males by 22 weeks of age) [42 (link),43 (link),44 (link)]. Coxsackievirus B4 (CVB4) Edwards strain (GenBank: S76772.1) was used for this study (a generous gift from Roger Loria, Virginia Commonwealth University, Richmond, VA, USA). NOD mice were exposed at 10 weeks of age via intraperitoneal (IP) injection of 500,000 plaque-forming units (PFUs) of CVB4 or sterile phosphate-buffered saline (PBS) (#J373, VWR, Radnor, PA, USA) as a stress control. Body weight and blood glucose were measured once a week for the course of the study. A drop of blood was collected from the tip of the tail and applied to a handheld glucometer (Freestyle Freedom Lite Blood Glucose Monitoring System, Abbott Laboratories, Lake Bluff, IL, USA). Mice were considered to be diabetic with a non-fasting blood glucose level ≥ 250 mg/dL, and confirmed by glucosuria prior to euthanasia (#2806, Bayer Healthcare, Leverkusen, Germany).
8
NOD mice for AhR study
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NOD/ShiLtJ (NOD) mice were obtained from The Jackson Laboratory and maintained in the specific pathogen-free animal facility at Oregon State University. NOD.AhR-/- mice were generated by backcrossing B6.129-AHRtm1Bra/J onto the NOD/LtJ background for more than 13 generations. All experiments used female littermate-matched mice. All animal procedures were carried out following protocols approved by the Institutional Animal Care and Use Committee at Oregon State University.
9
Diabetes Monitoring in NOD Mice
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The NOD/ShiLtJ (NOD) mice were purchased from The Jackson Laboratory and grown in our animal facility under specific pathogen-free conditions. All animals had access to food and water ad libitum. Blood glucose of NOD females was monitored in tail blood samples at weekly intervals using a glucometer (Roche Diagnostics, Florence, SC, USA) starting at 10 weeks of age. Diabetes was defined as two consecutive blood glucose measurements above 200 mg/dL. All the procedures were conducted in accordance with the European Guidelines for Animal Welfare and were approved by the Institutional Ethical Committee of the Institute of Cellular Biology and Pathology “Nicolae Simionescu”, from Bucharest, Romania and by the National Sanitary Veterinary and Food Safety Authority (authorization no. 296/23.08.2016).
10
Oral Vaccination for Type 1 Diabetes
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Seven-week-old female NOD/ShiLtJ (NOD) mice (Jackson Laboratories, Bar Harbor, ME) were maintained under pathogen-free conditions and housed at the animal care facility at City of Hope National Medical Center. The study was approved by the Institutional Animal Care and Use Committee (IACUC# 18017). Eight-week-old mice were orally vaccinated with Salmonella containing plasmid for the expression of IL10 and TFGβ and Salmonella expressing autoantigen PPI in 200 ml of 5% sodium bicarbonate on days 0 and 7. Vaccinated animals were also treated with anti-CD3 mAb for five consecutive days (days -1 to 3). The control (vehicle) treatment was 200 ml of 5% sodium bicarbonate given orally. Stools were collected from vaccine-, and control- treated animals at pre, 3-, 7-, 14-, 30-day post-vaccination.
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