Anti smarcal1

Manufactured by Cell Signaling Technology

Anti-SMARCAL1 is a primary antibody that recognizes the SMARCAL1 (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A-like 1) protein. SMARCAL1 is a chromatin remodeling enzyme that plays a role in DNA repair and replication. This antibody can be used to detect and analyze the SMARCAL1 protein in various experimental applications.

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Lab products found in correlation

Anti atrx, by Cell Signaling Technology (2 mentions) Anti α tubulin, by Merck Group (1 mentions) Nupage, by Thermo Fisher Scientific (1 mentions) Anti daxx, by Cell Signaling Technology (1 mentions) Anti atrx, by Santa Cruz Biotechnology (1 mentions) Sc 55584, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Abcam (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions)

2 protocols using anti smarcal1

SDS-PAGE and Immunoblotting Protocol

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Cell were lysed
into 2% w/v SDS (50 mM Tris, pH 6.8). Lysates were separated using
discontinuous SDS-PAGE to resolve proteins of molecular weight <
200 kDa or tris-acetate gels (NuPAGE, Invitrogen) to resolve proteins
of molecular weight > 200 kDa. Antibodies used for immunoblotting
were anti-SMARCAL1 (1/100, D3P5I; Cell Signaling), anti-DAXX (1/1000,
25C12; Cell Signaling), anti-ATRX (1/1000, D1N2E; Cell Signaling),
anti-ATRX (1/100, sc-55584; Santa Cruz Biotechnology), anti-alpha-tubulin
(1/5000, DM1A; Sigma-Aldrich), and anti-GAPDH (1/5000, ab9485; Abcam).

Goncalves T., Zoumpoulidou G., Alvarez-Mendoza C., Mancusi C., Collopy L.C., Strauss S.J., Mittnacht S, & Tomita K. (2020). Selective Elimination of Osteosarcoma Cell Lines with Short Telomeres by Ataxia Telangiectasia and Rad3-Related Inhibitors. ACS Pharmacology & Translational Science, 3(6), 1253-1264.

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Western Blot Analysis of SMARCAL1 and ATRX

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Cells were lysed in protein-denaturing lysis buffer and protein was quantified using the BCA Protein Assay Kit (Pierce). Equal amounts of protein were loaded on SDS-polyacrylamide gels (3–8% Tris-Acetate for blots probing for ATRX, 4–12% bis-tris for all others), transferred to membranes, blocked, and blotted with antibodies. Antibodies used included anti-SMARCAL1 (Cell Signaling Technologies), anti-ATRX (Cell Signaling Technologies), anti-β-Actin (Cell Signaling Technologies), and anti-GAPDH (Santa Cruz Biotechnology) for equal loading control. Original blots are provided in Supplementary Figures 10–11.

Diplas B.H., He X., Brosnan-Cashman J.A., Liu H., Chen L.H., Wang Z., Moure C.J., Killela P.J., Loriaux D.B., Lipp E.S., Greer P.K., Yang R., Rizzo A.J., Rodriguez F.J., Friedman A.H., Friedman H.S., Wang S., He Y., McLendon R.E., Bigner D.D., Jiao Y., Waitkus M.S., Meeker A.K, & Yan H. (2018). The genomic landscape of TERT promoter wildtype-IDH wildtype glioblastoma. Nature Communications, 9, 2087.

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