Smart-Pool short interfering RNA (siRNA) for various cellular targets including β-AR2 and non-target control siRNA (C-siRNA), and Dharmafect-2 transfection reagent were purchased from Dharmacon Inc. (Dharmacon-GE Life Sciences, Lafayette, CO). Recombinant chemokines and growth factors (Human SDF-1α, CXCL11, IL-8, EGF and amphiregulin) were purchased from R&D Systems (Minneapolis, MN). Full-length CXCR7 cDNA was obtained from Origene and was cloned in Ampicillin vectors for stable transfection in RWPE-1 cells. CXCR7-GFP and β-AR2 expression vectors were a gift from Dr. Kathleen Luker, University of Michigan. HuSH /shRNA Plasmid Panels for transient or stable down regulation of β-AR2 (GI326261 (1 (link)), GI326262 (2 (link)), (GI326263 (3 (link)), GI326264 (4 (link))) and scrambled shRNA (TR30013 (13 )) were purchased from Origene (Rockville, MD).
Human sdf1 α
Manufactured by R&D Systems
Human SDF1-α is a recombinant protein that represents the mature form of the human stromal cell-derived factor 1 alpha (SDF1-α) protein. SDF1-α is a chemokine that plays a role in cell migration and homing. It is the natural ligand for the CXCR4 receptor.
Automatically generated - may contain errors
Lab products found in correlation
Glass bottom dishes and plates, by MatTek (2 mentions)
Cytochalasin d, by Santa Cruz Biotechnology (2 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (2 mentions)
Dharmafect 2 transfection reagent, by Horizon Discovery (1 mentions)
Amphiregulin, by R&D Systems (1 mentions)
Cxcl11, by R&D Systems (1 mentions)
Hush shrna plasmid panels, by OriGene (1 mentions)
Epidermal growth factor (egf), by R&D Systems (1 mentions)
Oxyfluor, by Thermo Fisher Scientific (1 mentions)
Blebbistatin, by Abcam (1 mentions)
Anti cd43, by Thermo Fisher Scientific (1 mentions)
Alexa 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Nucleofector kit 5, by Lonza (1 mentions)
3 protocols using human sdf1 α
1
Knockdown of β-AR2 and CXCR7 in RWPE-1 Cells
2
Adhesion Molecule Purification and Assay
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Human SDF1-α was from R&D Systems. Wild-type soluble ICAM-1 (D1–D5) was expressed in 293T cells and purified on NiNTA agarose4 (link). OxyFluor was from Fisher. Cytochalasin D was from Santa Cruz. Glass-bottom dishes and plates were from Mattek. Leibovitz's L-15 medium was from Life Technologies. The reagents for the lentiviral Gateway system were from Invitrogen. Monoclonal antibodies used were TS1/18, KIM127 and m24, see also antibody staining section.
3
Purification of Soluble ICAM-1 from 293 Cells
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Wild-type soluble ICAM-1-His6 (D1-D5) was expressed in 293 cells and purified on Ni-NTA agarose3 (link). Human SDF1-α was from R&D System. Cytochalasin D was from Santa Cruz. Blebbistatin was from AbCam. Phalloidin-Alexa488 was from Invitrogen. Anti-CD43 was from ebiosciences. Glass-bottom dishes and plates were from Mattek. Leibovitz’s L-15 medium and RPMI-1640 medium were from Life Technologies. The reagents for the lentiviral Gateway system were from Life Technologies. Nucleofector Kit V was from Lonza.
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