K2edta treated tubes

Manufactured by BD

K2EDTA-treated tubes are laboratory specimen collection tubes that contain the anticoagulant potassium EDTA. These tubes are used to collect and store blood samples for various laboratory analyses, such as complete blood count (CBC) and other hematological tests.

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Lab products found in correlation

Cd3ε pe cy7 clone 145.2c11, by BioLegend (1 mentions) Cd45.2 apc clone 104, by BioLegend (1 mentions)

Close spelling variants of this product

K2EDTA (197 citations) K2EDTA tubes (66 citations)

4 protocols using k2edta treated tubes

Plasma Metabolomic Analysis of TAVR

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Blood sample was obtained from peripheral vein before valve deployment during the index TAVR procedure and at the 7th day after TAVR (normally the day of discharge) in K2EDTA-treated tubes (BD). Subsequently, these blood samples were centrifuged at 2000 g for 10 min to pellet the cellular elements. The supernatant plasma was stored at − 80 °C until sample preparation for liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) analysis.

Xiong T.Y., Liu C., Liao Y.B., Zheng W., Li Y.J., Li X., Ou Y., Wang Z.J., Wang X., Li C.M., Zhao Z.G., Feng Y., Liu X.J, & Chen M. (2020). Differences in metabolic profiles between bicuspid and tricuspid aortic stenosis in the setting of transcatheter aortic valve replacement. BMC Cardiovascular Disorders, 20, 229.

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Isolation and Analysis of Murine Myeloid Cells

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For analysis of circulating CD11b⁺Gr1⁺ cells, 100 μl of murine peripheral blood was collected once per week by inserting a 26-G needle into the lateral tail vein to collect blood into a heparin-coated capillary tube. Blood samples were transferred to K₂EDTA-treated tubes (BD Microtainer, Franklin Lakes, NJ), centrifuged at 1000×g for 10 min at room temperature, and the plasma removed. The cellular fraction of each sample was treated with NH₄Cl for 9 min on ice to induce erythrocyte lysis prior to antibody incubation for subsequent flow cytometry analyses. For antibody array or enzyme-linked immunosorbent assay (ELISA) analyses, plasma was collected by terminal cardiac puncture using a heparin-coated syringe with a 26-G needle prior to processing as outlined above.

Bosiljcic M., Cederberg R.A., Hamilton M.J., LePard N.E., Harbourne B.T., Collier J.L., Halvorsen E.C., Shi R., Franks S.E., Kim A.Y., Banáth J.P., Hamer M., Rossi F.M, & Bennewith K.L. (2019). Targeting myeloid-derived suppressor cells in combination with primary mammary tumor resection reduces metastatic growth in the lungs. Breast Cancer Research : BCR, 21, 103.

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Quantification of Antigen-Specific CD8+ T Cells

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On day 20 after immunizations, whole blood was collected in K₂EDTA treated tubes (BD Biosciences), treated with ACK lysis buffer (KD Medical), washed, resuspended in cold FACS buffer (PBS supplemented with 2% FBS and 50 μM dasatinib), and plated in a 96-well U-bottom plate. Next, the cells were centrifuged for 5 min at 1,500 rpm and resuspended in FACS buffer and incubated with Fc-block (anti-CD16/CD32, clone 2.4G2; Tonbo) for 15 min at 4°C, and stained with antibodies CD45.2 (APC; clone 104; BioLegend), CD3ε (PE/Cy7; clone 145.2C11; BioLegend), and CD8α (APC/Cy7; clone 53–6.7; Tonbo) for 1 h at 4°C. Cells were then washed 3x in FACS buffer and then stained for 2 h with 1.5 μg/mL of PE-labeled OVA _257–264 pOVA/H-2K^b tetramer prepared according to a previously reported procedure.^{59 (link)} Cells were then washed 3x in FACS buffer, resuspended using FACS buffer supplemented with 1 μg/mL DAPI, and analyzed using an Amnis CellStream Luminex flow cytometer. Representative flow cytometry data and gating strategies for determining the frequency of SIINFEKL-specific CD8⁺ T cells are shown in Supplementary Figure 3.

Carson C.S., Becker K.W., Garland K.M., Pagendarm H.M., Stone P.T., Arora K., Wang-Bishop L., Baljon J.J., Cruz L.D., Joyce S, & Wilson J.T. (2022). A Nanovaccine for Enhancing Cellular Immunity via Cytosolic Co-Delivery of Antigen and PolyIC RNA. Journal of controlled release : official journal of the Controlled Release Society, 345, 354-370.

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Murine Hyperglycemia Blood Profiling

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Peripheral and whole blood samples were collected from mouse groups via tail vein cutting and cardiac puncture into K₂EDTA-treated tubes (BD Biosciences), respectively, 30 and 60 days after hyperglycemia induction. Levels of circulating granulocytes, lymphocytes, RBC, and white blood cells (WBC) were analyzed using an automated blood cell counter (Sysmex XE-2100; TOA Medical Electronics Co., Kobe, Japan).

Bhattarai G., So H.S., Kim T.G., Kieu T.T., Kim Y.W., Yang K.R., Lee J.C., Kook S.H, & Jeon Y.M. (2022). Astaxanthin Protects against Hyperglycemia-Induced Oxidative and Inflammatory Damage to Bone Marrow and to Bone Marrow-Retained Stem Cells and Restores Normal Hematopoiesis in Streptozotocin-Induced Diabetic Mice. Antioxidants, 11(12), 2321.

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