A multiplexed immunohistochemistry (mIHC) analysis was performed on formalin‐fixed paraffin‐embedded (FFPE) tissue slides using the Mantra multispectral imaging platform (Akoya Biosciences, Marlborough, Massachusetts). FFPE slides were stained with anti‐CD8 (clone C8/144B, Thermo Fisher Scientific), anti‐CD68 (clone PG‐M1, Dako Agilent, Santa Clara, California), anti‐HO‐1 (clone HO‐1‐1, Thermo Fisher Scientific) and anti‐glial fibrillary acidic protein (GFAP, clone EP672Y, Abcam, Cambridge, UK) antibodies. DAPI (Akoya Biosciences) was used as a nuclear counterstain. The autofluorescence background signal was subtracted by using an unstained control slide processed in parallel. The Mantra multispectral imaging platform (Akoya Biosciences) was employed for image acquisition at ×20 magnification and data analysis was carried out with InForm 2.4.1 software (Akoya Biosciences).
Mantra multispectral imaging platform
Manufactured by Akoya Biosciences
Sourced in New Zealand
The Mantra multispectral imaging platform is a laboratory instrument designed for the acquisition and analysis of multispectral images. It is capable of capturing images across multiple wavelengths, allowing for the detection and quantification of various biological markers within samples.
Automatically generated - may contain errors
2 protocols using mantra multispectral imaging platform
1
Multiplexed Immunohistochemistry for Tissue Analysis
2
Multispectral Imaging of FFPE Samples
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Formalin-Fixed Paraffin-Embedded (FFPE) specimens from one patient both at diagnosis and recurrence were analyzed by multispectral imaging. FFPE slides were stained with anti-CD8 (clone C8/144B, Thermo Fisher Scientific, Waltham, MA, USA), anti-CD68 (clone PG-M1, Dako Agilent, Santa Clara, CA, USA) and anti-glial fibrillary acidic protein (GFAP, clone EP672Y, abcam, Cambridge, UK) antibodies for T cell, macrophage and surrounding tissue detection, respectively. DAPI (Akoya Biosciences, Marlborough, MA, USA) was used as a nuclear counterstain. The autofluorescence background signal was subtracted using an unstained control slide processed in parallel. The Mantra multispectral imaging platform (Akoya Biosciences) was employed for image acquisition at 20× magnification and data analysis was carried out with InForm 2.4.1 software (Akoya Biosciences). The immune cell infiltrate was quantified as cell density/megapixel.
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