After cells were incubated with the inhibitors, respectively, for 12 h, Caveolin-1 enriched membrane fractions were isolated as described above and the final samples were detected by western blotting. The protein samples were separated by SDS-PAGE gel and then electrotransferred to PVDF membranes. Subsequently, blots were subjected to immunostaining with antibodies against Caveolin-1 (Cell Signaling Technology, 1 : 800), Cavin-1 (ANBO, 1 : 500), and lectin-like oxLDL receptor (Lox-1, WuXi AppTec, 1 : 1000). After incubation for 1 h with a peroxidase-conjugated secondary antibody (Abbkine, 1 : 10000), bands were visualized by an ECL western blotting detection system (NDR, Israel). The band intensities were quantified using ImageJ software.
Cavin 1
Manufactured by WuXi AppTec
Cavin-1 is a laboratory equipment designed for sample preparation and handling. It is a multi-functional device that can perform various tasks, such as vortexing, mixing, and shaking, to facilitate efficient sample processing in a laboratory setting. The core function of Cavin-1 is to provide a reliable and consistent method for mixing and agitating samples, ensuring homogeneous solutions and aiding in the preparation of samples for further analysis.
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2 protocols using cavin 1
1
Caveolin-1 Enrichment and Western Blotting
2
Caveolin-1 Enrichment and Western Blotting
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After cells were incubated with the inhibitors, respectively, for 12 h, Caveolin-1 enriched membrane fractions were isolated as described above and the final samples were detected by western blotting. The protein samples were separated by SDS-PAGE gel and then electrotransferred to PVDF membranes. Subsequently, blots were subjected to immunostaining with antibodies against Caveolin-1 (Cell Signaling Technology, 1 : 800), Cavin-1 (ANBO, 1 : 500), and lectin-like oxLDL receptor (Lox-1, WuXi AppTec, 1 : 1000). After incubation for 1 h with a peroxidase-conjugated secondary antibody (Abbkine, 1 : 10000), bands were visualized by an ECL western blotting detection system (NDR, Israel). The band intensities were quantified using ImageJ software.
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