Dau5884

Manufactured by Bio-Techne

Sourced in United Kingdom, United States

DAU5884 is a laboratory equipment product from Bio-Techne. It is a device designed for scientific applications, but a detailed unbiased and factual description is not available at this time.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using dau5884

Synthesis and Characterization of Muscarinic Receptor Agonists

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carbachol (CCh), CGP55845, NBQX, DCG-IV, D-APV, picrotoxin, atropine, mecamylamine, nitrocaramiphen, DAU5884, AM251, GDP-β-S were purchased from Tocris (UK). GSK-5 was synthesised in-house at Eli Lilly and Co. Stock solutions of these compounds were made by dissolving in water. The selective muscarinic M₁ and M₄ receptor agonist Compound 1 was synthesised in-house at Sosei Heptares and dissolved in DMSO for stock solution. The purity of the final compounds was determined by HPLC or LC/MS analysis to be >95%. Additional experimental details relating to the synthesis of Compound 1 and associated structures are described in detail in WO2015/118342 which relates to the invention of agonists of the muscarinic M₁ receptor and/or M₄ receptor and which are useful in the treatment of muscarinic M₁/M₄ receptor-mediated diseases.

Palacios-Filardo J., Udakis M., Brown G.A., Tehan B.G., Congreve M.S., Nathan P.J., Brown A.J, & Mellor J.R. (2021). Acetylcholine prioritises direct synaptic inputs from entorhinal cortex to CA1 by differential modulation of feedforward inhibitory circuits. Nature Communications, 12, 5475.

+ Open protocol

+ Expand

Perfusion of Tissues for Imaging and Electrophysiology

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues used for imaging and electrophysiological experiments were perfused with KRB containing (mmol/L): NaCl, 120.35; KCl, 5.9; NaHCO₃, 15.5; NaH₂PO₄, 1.2; MgCl₂, 1.2; CaCl₂, 2.5; and glucose, 11.5. KRB was bubbled with a mixture of 97% O₂ – 3% CO₂ and warmed to 37 ± 0.2 °C. Atropine, neostigmine, N_ω-nitro-L-arginine (L-NNA) and carbachol (CCh) were purchased from Sigma-Aldrich (St Louis, MO, USA). Tetrodotoxin (TTX), Ani9, cyclopiazonic acid (CPA), RP 67580, Substance P, GR 73632, AF-DX 116, DAU 5884, MEN 10376 and MRS 2500 were purchased from Tocris Bioscience (Ellisville, Missouri, USA). GSK 7975A was purchased from Aobious. All drugs were dissolved as recommended by the manufacturers and then diluted to the desired concentrations with KRB solution.

Drumm B.T., Rembetski B.E., Huynh K., Nizar A., Baker S.A, & Sanders K.M. (2020). Excitatory cholinergic responses in mouse colon intramuscular interstitial cells of Cajal are due to enhanced Ca2+ release via M3 receptor activation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(8), 10073-10095.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!