HEK293 cells were transfected with 5 μg of (Myc-DDK-tagged)-Human CD59 plasmid (OriGene) and 2.5 μl of EndoFectin Plus transfection reagent (GeneCopoeia) in a confluent 35-mm plate according to the manufacturer's protocol. After transfection for 72 hours, the HEK293 cells were lysed with immunoprecipitation (IP) lysis buffer (Thermo Scientific). The expression of Myc-DDK–tagged human CD59 was confirmed by detecting DDK with anti-DDK monoclonal antibody (OriGene) by Western blotting.
Myc ddk tagged human cd59 plasmid
Manufactured by OriGene
The Myc-DDK-tagged)-Human CD59 plasmid is a laboratory tool designed to express the human CD59 protein in cells. The plasmid contains the coding sequence for the CD59 protein with Myc and DDK tags added to the protein. This plasmid can be used for various research applications involving the study of the CD59 protein.
Automatically generated - may contain errors
2 protocols using myc ddk tagged human cd59 plasmid
1
Overexpression of Human CD59 in HEK293 Cells
2
Overexpression of Human CD59 in HEK293 Cells
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