Ripa lysis buffer p0013

Iso (CS-2582) was purchased from MCE (Med Chem Express), and CGA (C109404) was purchased from United States, Med Chem Express, Inc., Ltd (Princeton, NJ, United States) (HY-N0055) (isolated purity ≥ 99.5; CGA ≥ 99.5). IM-12 was purchased from United States, Med Chem Express, Inc. The anti-ANP antibody (ab189921) was purchased from Abcam, Inc. (Cambridge, United Kingdom) , and the anti-β-catenin (cat. no. 8480), anti-c-Myc (cat. no. 5605), anti-LRP6 (cat. no. 3395), anti-glycogen synthase kinase-3β (GSK-3β; cat. no. 12456), and anti-p-GSK-3β (cat. no. 9322) antibodies to analyze the Wnt/β-catenin signaling pathway were purchased from United States, Cell Signaling, Inc., Ltd (Beverl, MA, United States). The bicinchoninic acid (BCA) protein quantification kit (P0010) and the Actin-Tracker Green kit (C1033) were purchased from China, Beyotime Biotechnology, Co., Ltd (Shanghai, China). The anti-GAPDH antibody (BM1623) and the horseradish peroxidase-conjugated secondary antibody (BA1054; BM2002) were purchased from China, Wuhan Boster Biotechnology Co., Ltd (Wuhan, China). The RIPA lysis buffer (P0013) was purchased from Beyotime Biotechnology, Ltd (Shanghai, China). The Cell Counting Kit-8 (40203ES76) was purchased from China, Yeasen Biotechnology, Co., Ltd (Shanghai, China).

Formononetin (For, A0232), isorhamnetin (Iso, A0514), kaempferol (Kae, A0129), calycosin (Cal, A0190), and quercetin (Que, A0083) (purity ≥ 98%) were provided by Chengdu Must Biotechnology Co., Ltd. (Chengdu, China), which were mixed with dimethyl sulfoxide (DMSO, 0.1%, v/v; Sigma, St. Louis, MO, United States). In the meantime, the HEK293-derived recombinant human TGF-β1 (100-21) was purchased from PeproTech (New Jersey, United States). The anti-α-SMA (14395-1-AP) and anti-GAPDH (6004-1-lg) antibodies, together with the HRP-labeled AffiniPure goat anti-mouse IgG antibody (H + L) (SA00001-1), were provided by Proteintech (Wuhan, China). The HRP-labeled goat anti-rabbit IgG antibody (H + L) (BS13278) was provided by Bioworld Technology (St. Paul, MN, United States). The Alexa fluor 568-conjugated goat anti-rabbit IgG (H + L) antibody was purchased from Abcam (Cambridge, MA, United States). The enhanced BCA protein assay kit (P0010), RIPA lysis buffer (P0013) and DAPI staining solution (C1005) were provided by Beyotime Institute of Biotechnology (Shanghai, China).