Serum IgG and mucosal secretory IgA (sIgA) concentrations were measured using ELISA. Before testing, the samples were briefly diluted with PBS. Microplates containing 100 µL of diluted samples and 5 µg/well of H. pylori lysates were coated overnight at 4 °C. HRP-conjugated goat anti-mouse IgG (31,430, Thermo Fisher) and sIgA (ab97235, Abcam) were used after the plate had been cleaned with PBST. Tetramethylbenzidine (Solarbio, China) was used to view the plates after washing for 15 min in complete darkness. Finally, a solution of 2 M sulfuric acid (Solarbio, China) was used to terminate the process. A microplate reader was used to measure the absorbance at 450 nm.
Hrp conjugated goat anti mouse igg 31 430
Manufactured by Thermo Fisher Scientific
HRP-conjugated goat anti-mouse IgG (31,430) is a secondary antibody conjugated to horseradish peroxidase (HRP). It is designed for use in immunoassays and other immunochemical applications that require the detection of mouse immunoglobulin G (IgG).
Automatically generated - may contain errors
Lab products found in correlation
Ab97235, by Abcam (1 mentions)
Sulfuric acid, by Solarbio (1 mentions)
Tetramethylbenzidine, by Solarbio (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Anti tubulin t9026, by Merck Group (1 mentions)
Anti gm130 ab52649, by Abcam (1 mentions)
Anti sec31a, by BD (1 mentions)
Dc protein assay 500 0119, by Bio-Rad (1 mentions)
Clarity western ecl substrate 170 5061, by Bio-Rad (1 mentions)
Goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Effectene transfection reagent, by Qiagen (1 mentions)
2 protocols using hrp conjugated goat anti mouse igg 31 430
1
Serum IgG and Mucosal sIgA Quantification
2
Comprehensive Reagents and Antibody Protocol
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Reagents and antibodies were purchased from the following suppliers: HRP–conjugated goat anti-mouse IgG (31430) and goat anti-rabbit IgG (31461); Alex Fluor 488 goat anti-mouse IgG (A11029), Texas Red goat anti-mouse IgG (T-862), and goat anti-rabbit IgG (T-2767); and Alexa Fluor Plus 647 goat anti-mouse IgG (A32728) and goat anti-rabbit IgG (A32733) are from Thermo Fisher Scientific. Anti-beclin-1 (NB500-249), anti-FAM134B (NBP2-55248), and anti-Sec62 (NBP1-84045) are from Novus Biologicals. Anti-calnexin (C4731), anti-HA (H9658), anti-LC3B (L7543), anti-Myc (C3956), and anti-tubulin (T-9026) are from Sigma-Aldrich. Anti-ERGIC53 (ALX-804-602) is from Enzo Life Sciences. Anti-GABARAP+L1+L2 (ab109364) and anti-GM130 (ab52649) are from Abcam. Anti-LAMP2 (555803) and anti-Sec31A (612351) are from BD Pharmingen. Anti-p62 (H00008878-M0162) is from Abnova. Anti-VMP1 (12978s) is from Cell Signaling. DC Protein Assay (500-0119) and Clarity Western ECL Substrate (170-5061) are from Bio-Rad. Effectene Transfection Reagent (301427) is from Qiagen. Lipofectamine RNAiMAX Reagent (100014472) is from Thermo Fisher Scientific. MTS cross-linking reagents M3M (1,3-propanediyl bis-MTS) and M8M (1,5-pentanediyl bis-MTS) are from Toronto Research Chemicals. ProLong Diamond antifade mountant without (P36970) or with DAPI (P36971) is from Thermo Fisher Scientific.
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