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Anti goat af 568

Manufactured by Thermo Fisher Scientific
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Anti-goat AF-568 is a fluorescently labeled secondary antibody used in immunofluorescence assays. It is conjugated to the Alexa Fluor 568 dye, which has an excitation maximum at 578 nm and an emission maximum at 603 nm.

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Lab products found in correlation

Lsm800 confocal microscope, by Zeiss (4 mentions) Zen blue software, by Zeiss (4 mentions) Mouse anti muc5ac, by Thermo Fisher Scientific (2 mentions) Goat anti hpiv3, by Abcam (2 mentions) Anti β tubulin 647 antibody, by Novus Biologicals (2 mentions) Mouse anti zo 1, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Secondary goat anti-mouse AF-568 Goat anti-mouse IgG (H + L) AF 568

2 protocols using anti goat af 568

1

3D Culture Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
3D cultures were fixed with 4% paraformaldehyde-PBS for 20 minutes followed by permeabilization with 0.5 % TritonX-100-PBS for 2 hours. Nonspecific protein binding sites were blocked with 5% BSA-PBS for 1 hour. HPIV3 was detected using goat anti-HPIV3 (Abcam) followed by anti-goat AF-568 (Thermo Scientific). Muc5AC was detected using mouse anti-Muc5AC (Thermo Scientific). ZO-1 (tight junctions) was detected using mouse anti-ZO-1 (BD Biosciences; 610966). Anti-mouse-FITC secondary antibody (SantaCruz) was used for both Muc5AC and ZO-1 staining. Anti-β-tubulin-647 antibody (Novus Biologicals) was used for detection of β-tubulin. Membranes were placed on glass slides using a mounting medium supplemented with 0.1 mM 4,6-diamidino-2-phenylindole (DAPI; Molecular Probes), covered with a coverslip, and edges sealed with nail polish. A Zeiss LSM 800 confocal microscope coupled with AiryScan module was used for detection, Zeiss Zen Blue software was employed for image analysis.
Cox R.M., Sourimant J., Toots M., Yoon J.J., Ikegame S., Govindarajan M., Watkinson R.E., Thibault P., Makhsous N., Lin M.J., Marengo J.R., Sticher Z., Kolykhalov A.A., Natchus M.G., Greninger A.L., Lee B, & Plemper R.K. (2020). Orally Efficacious Broad-Spectrum Allosteric Inhibitor of Paramyxovirus Polymerase. Nature microbiology, 5(10), 1232-1246.
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2

3D Culture Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
3D cultures were fixed with 4% paraformaldehyde-PBS for 20 minutes followed by permeabilization with 0.5 % TritonX-100-PBS for 2 hours. Nonspecific protein binding sites were blocked with 5% BSA-PBS for 1 hour. HPIV3 was detected using goat anti-HPIV3 (Abcam) followed by anti-goat AF-568 (Thermo Scientific). Muc5AC was detected using mouse anti-Muc5AC (Thermo Scientific). ZO-1 (tight junctions) was detected using mouse anti-ZO-1 (BD Biosciences; 610966). Anti-mouse-FITC secondary antibody (SantaCruz) was used for both Muc5AC and ZO-1 staining. Anti-β-tubulin-647 antibody (Novus Biologicals) was used for detection of β-tubulin. Membranes were placed on glass slides using a mounting medium supplemented with 0.1 mM 4,6-diamidino-2-phenylindole (DAPI; Molecular Probes), covered with a coverslip, and edges sealed with nail polish. A Zeiss LSM 800 confocal microscope coupled with AiryScan module was used for detection, Zeiss Zen Blue software was employed for image analysis.
Cox R.M., Sourimant J., Toots M., Yoon J.J., Ikegame S., Govindarajan M., Watkinson R.E., Thibault P., Makhsous N., Lin M.J., Marengo J.R., Sticher Z., Kolykhalov A.A., Natchus M.G., Greninger A.L., Lee B, & Plemper R.K. (2020). Orally Efficacious Broad-Spectrum Allosteric Inhibitor of Paramyxovirus Polymerase. Nature microbiology, 5(10), 1232-1246.
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