Caffeine, an ATM kinase specific inhibitor, okadaic acid for inhibiting PP2A, 3,3’-iminodipropionitrile (IDPN) for vimentin disruption, and propidium iodide (PI) for cell staining were purchased from Sigma-Aldrich (St. Louis, USA). Proteasome inhibitor MG132 was from Calbiochem (San Diego, USA). Etoposide was used to stimulate DNA damage, while nocodazole was used to arrest cells in M phase. These chemicals were from EMD Biosciences, Inc. (San Diego, USA). The p17 monoclonal antibodies were produced previously by our laboratory. Rabbit anti-p-ATM (Ser 1981), rabbit anti-ATM, mouse anti-Chk1, mouse anti-p-Chk1, mouse anti-Chk2, mouse anti-p-Chk2, rabbit anti-CDC25C, mouse anti-CDK1, rabbit anti-p-vimentin (Ser 56), rabbit anti-p-vimentin (Ser 82), rabbit anti-vimentin, rabbit anti-p-p53 (Ser 15), rabbit anti-p53, rabbit anti-p-Plk1 (T210), rabbit anti-Plk1, rabbit anti-Myt1 were from Cell Signaling Technology (Danvers, USA). Rabbit anti-beta-actin antibody was from Millipore (Billerica, MA). Rabbit anti-p-CDC25C (Ser 216), rabbit anti-p-CDK1 (T14 and Y15), rabbit anti-nucleoporin Tpr, rabbit anti-p-p21 (T145), rabbit anti-p21 were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-p-Myt1 (T495) antibody was from Sigma-Aldrich. Anti-mouse IgG (H+L) and anti-rabbit IgG (H+L) antibodies were purchased from Kirkegard & Perry Laboratories (Washington, DC., USA).
Chiu H.C., Huang W.R., Liao T.L., Wu H.Y., Munir M., Shih W.L, & Liu H.J. (2016). Suppression of Vimentin Phosphorylation by the Avian Reovirus p17 through Inhibition of CDK1 and Plk1 Impacting the G2/M Phase of the Cell Cycle. PLoS ONE, 11(9), e0162356.
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