Preservative-free 2 % lidocaine (31802232) was purchased from Tianjin Shuicheng Pharmaceutical limited by share Ltd, China, while Penicillin and streptomycin were purchased from TransGen Biotech, China. DMEM/F12, DMEM, horse serum, and fetal bovine serum were purchased from Gibco. DMSO, Retigabine, NaCl, CsF, CsCl, MgCl2, CaCl2, KCl, Glucose, HEPES, EGTA, and KF were purchased from Sigma-Aldrich, whereas Capsaicin was purchased from MedchemExpress, China. Stock solutions of lidocaine or Retigabine were diluted or dissolved in DMSO to prepare a 100 mM storage solution, and an extracellular gradient dilution solution was used to obtain the detection concentration for the experiment. A stock solution of Capsaicin was also dissolved in DMSO to prepare a 300 μM storage solution and extracellular gradient dilution was used to obtain the detection concentration for the experiment.
Capsaicin
Manufactured by MedChemExpress
Sourced in China
Capsaicin is a naturally occurring chemical compound found in various pepper plants. It is a colorless, crystalline substance that exhibits pungent, spicy characteristics. Capsaicin is commonly used in scientific research and laboratory settings as a chemical reagent.
Automatically generated - may contain errors
Lab products found in correlation
Glucose, by Merck Group (2 mentions)
Capsazepine, by MedChemExpress (2 mentions)
Penicillin, by Transgene (1 mentions)
Streptomycin, by Transgene (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Retigabine, by Merck Group (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hepes, by Merck Group (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Cacl2, by Merck Group (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
Mcc950, by MedChemExpress (1 mentions)
Piperine, by MedChemExpress (1 mentions)
Ionomycin, by MedChemExpress (1 mentions)
7 protocols using capsaicin
1
Lidocaine and Retigabine Preparation
2
Subarachnoid Hemorrhage Model and TRPV1 Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6 mice (male, 22–25 g) were purchased from Vital River Laboratory Animal Technology (Beijing, China). The procedures involved in mice were conformed to the guidelines of the National Institutes of Health on the care and use of laboratory animals and approved by the Institutional Animal Care and Use Committee of Shandong First Medical University.
SAH model was constructed via endovascular perforation as the previous study [28 (link)]. Briefly, the mice were anesthetized using 5% isoflurane, and then the anesthesia was maintained at 2% isoflurane during the entirety of the experiment, the nylon suture was passed through the external carotid artery to the bifurcation of the anterior and middle artery and ultimately punctured to cause blood to flow into the subarachnoid space.
Mice were injected with 30 mg/kg capsazepine (CPZ, MedChemExpress, Cat#HY-15640) subcutaneously or 10 mg/kg capsaicin (CAP, MedChemExpress, Cat# HY-10448) intraperitoneally post-modeling to inhibit or active TRPV1 [26 (link), 29 (link)], respectively. MCC950 (MedChemExpress, Cat# HY-12815) (40 mg/kg) were intraperitoneally injected post-modeling and 12 h later [8 (link)]. The drugs above were all purchased from MedChemExpress (NJ, USA).
SAH model was constructed via endovascular perforation as the previous study [28 (link)]. Briefly, the mice were anesthetized using 5% isoflurane, and then the anesthesia was maintained at 2% isoflurane during the entirety of the experiment, the nylon suture was passed through the external carotid artery to the bifurcation of the anterior and middle artery and ultimately punctured to cause blood to flow into the subarachnoid space.
Mice were injected with 30 mg/kg capsazepine (CPZ, MedChemExpress, Cat#HY-15640) subcutaneously or 10 mg/kg capsaicin (CAP, MedChemExpress, Cat# HY-10448) intraperitoneally post-modeling to inhibit or active TRPV1 [26 (link), 29 (link)], respectively. MCC950 (MedChemExpress, Cat# HY-12815) (40 mg/kg) were intraperitoneally injected post-modeling and 12 h later [8 (link)]. The drugs above were all purchased from MedChemExpress (NJ, USA).
3
Potent Compound Characterization Protocol
5
TRPV4-Mediated Inflammation Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All salts were supplied by Sangon Biotech. Glucose, caffeine, carbachol, and indomethacin were purchased from Sigma-Aldrich. Capsaicin, ouabain, SB705498, RN1734, RN1747, U73343, HC067047, GSK1016790A, methyl arachidonyl fluorophosphonate, AA, miconazole, and nystatin were purchased from MedChemExpress (MCE; Monmouth Junction). DSS was from MP Biomedicals (MP Bio). U73122 was from Tocris Bioscience (Tocris). Amiloride hydrochloride was from Molecular Probes, Inc (Molecular Probes). TNF-α and Fura-2/AM (Cat# F1221, Invitrogen) was from Invitrogen. TRPV4 (Abcam Cat# ab39260, RRID: AB_1143677 ), alpha 1 Sodium Potassium ATPase (phospho Y10) (Abcam Cat# ab124677, RRID: AB_10974461 ), Alexa Fluor 488 labeled anti-rabbit (Abcam Cat# ab150077, RRID: AB_2630356 ) antibodies, and MPO Assay Kit were from Abcam. Anti-GAPDH (Proteintech Cat# 60004-1-Ig, RRID: AB_2107436 ) was from Proteintech.
6
Hyperoxia effects on A549 cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human alveolar A549 cells were purchased from Haixing Biological Technology Co., Ltd. (cat. no. TCH-C116). Cells were cultured in DMEM-HIGH Glucose medium supplemented with 10% fetal bovine serum (AusGeneX Pty Ltd.), 100 U/ml penicillin and 0.1 mg/ml streptomycin (Beyotime Institute of Biotechnology). The cells were plated in 6- or 96-well plates for subsequent experiments. When the cell confluence reached 50–60%, the cells were treated with 10 nM CGRP (cat. no. HY-P1548A; MedChemExpress) or 1 µM capsaicin (cat. no. HY-10448; MedChemExpress), and then stimulated with hyperoxia. Cells were pretreated with CGRP8-37 (cat. no. HY-P1014), SB-705498 (cat. no. HY-10633), BAPTA-AM (cat. no. HY-100545), U-73122 (cat. no. HY-13419), U-73343 (cat. no. HY-108630), or Go6976 (cat. no. HY-10183; all from MedChemExpress) for 2 h before CGRP treatment. Hyperoxia exposure was defined as cells cultured in a closed oxygen chamber (Billups-Rothenberg, Inc.) supplied with 95 O2 and 5% CO2 for 24 h (31 (link)). The cells of the control group were cultured under normal air conditions. All cells were cultured at a constant temperature of 37°C and supplied with 5% CO2.
7
Capsaicin-Induced Apoptosis Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Capsaicin was purchased from MedChemexpress (Cat. No.: HY-10448; Purity: 99.01%; Manmouth Junction, NJ, USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). Annexin V-APC/7-AAD apoptosis detection kit was purchased from BD Biosciences (San Diego, CA, USA). TUNEL assay kit was purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). The primary antibodies against FBI-1 and GAPDH were obtained from Abcam (Cambridge, MA, USA). The primary antibodies against Ki-67, Bcl-2, Bax and Lamin B were obtained from Wanlei Biotechnology Co., Ltd. (Shenyang, China). The primary antibodies against cleaved-Caspase 3, Survivin and NF-κB p65 were obtained from Cell Signaling Technology (Danvers, MA, USA). The HRP-conjugated anti-rabbit IgG secondary antibody was obtained from EarthOx Life Sciences (Millbrae, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!