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Flag polyclonal

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FLAG polyclonal is a laboratory reagent used to detect and purify proteins in biological samples. It is a polyclonal antibody that recognizes the FLAG epitope, a small peptide tag that can be added to recombinant proteins for identification and purification purposes. The FLAG polyclonal antibody can be used in various applications, such as Western blotting, immunoprecipitation, and affinity chromatography.

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Lab products found in correlation

Actin, by Merck Group (2 mentions) Sodium molybdate, by Merck Group (2 mentions) Horseradish peroxidase conjugated goat anti mouse or rabbit igg, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 488 goat anti mouse igg h l antibody, by Thermo Fisher Scientific (2 mentions) Androgen receptor, by Santa Cruz Biotechnology (2 mentions) Flag m2 monoclonal, by Merck Group (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) P myc, by Cell Signaling Technology (1 mentions) Ubqln4, by Santa Cruz Biotechnology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Flag m2 conjugated agarose beads, by Merck Group (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Wnt5a b, by Cell Signaling Technology (1 mentions)

5 protocols using flag polyclonal

1

Comprehensive Immunoblotting Protocol for Cell Signaling

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UBQLN1 #14526, UBQLN2 #85509, Cyclin D1 #2978, Cyclin D3 #2936, Cyclin A2 #4656, Cyclin E1 #4129, Cyclin E2 #4132, Cyclin H #2927, Cyclin B1 #4138, CDK2 #2546, CDK4 #2906, CDK6 #3136, GAPDH #5174, c-MYC #9402, p-MYC #13748, MYCBP #517020, VDAC # 4866, CREB #9197, Cdc42 #2462, E-cadherin #3195, N-cadherin #13116, Snail #3879, Slug #9585, Zeb1 #3396, β-catenin #8480, Claudin1 #4933, GFP #2956, (Cell Signaling Technologies Inc. Danvers, MA, USA); Tubulin #B512, FLAG M2 conjugated agarose beads, FLAG poly-clonal #F7425, FLAG Peptide #F-3290 (Sigma-Aldrich, Inc. St. Louis, MO, USA); UBQLN3#376548, UBQLN4 #136145, β-actin #517582 (Santa Cruz Biotechnology, Inc. Dallas, TX, USA); Alexa Fluor 488 goat anti-rabbit IgG #A11034 (Molecular Probes, Invitrogen detection technologies, Eugene, OR, USA); Alexa Fluor 568 Phalloidin #A12380 (Life technologies, Eugene, OR, USA). Ubqln polyclonal was made by inoculating rabbits with a peptide specific to Ubqln1 (Yenzym Antibodies LLC, Brisbane, CA, USA).
Shah P.P, & Beverly L.J. (2023). UBQLN Family Members Regulate MYC in Lung Adenocarcinoma Cells. Cancers, 15(13), 3389.
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2

Signaling Pathway Antibody Analysis

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Cell Signaling Technology antibodies used: Wnt5a/b (#2530), Snail (#3879), Slug (#9585), ZEB1 (#3396), LKB1 (#3047), P-ACC S79 (#3661), Total ACC (#4190), Axin2 (#2151), MARK2 (#9118), MARK3 (#9311), AMPKalpha (#2532), P-ULK1 S555 (#5869), Nuak1 (#4458), SIK2 (#6919), GST (#2622), myc-tag (#2272), P-Src family Y416 (#2113), Src (#2109), P-Paxillin Y118 (#2541), Pathscan I for P-ERK1/2 and P-Akt S473 (#5301), Total ERK1/2 (#4695), P-S6K (#9234), HA-tag (#3724), P-MEK1/2 (#9154), P-p90RSK S380 (#11989), P-FAK Y925 (#3284). Epitomics antibodies used: Phospho-FAK Y397 (#2211-1), Phospho-FAK Y576/577 (#2183-1), Total FAK1 (#2146-1), Zyxin (#3586-1). BD Transduction Labs antibodies used: Paxillin (P13520). Sigma antibodies used: Actin (A5441), Flag polyclonal (F7425). Protein Tech antibodies used: MARK1 (21552-1-AP), MARK2 (15492-1-AP). Millipore antibodies used: ZEB2 (ABT332), MARK4 (07-699). Abcam antibodies used Twist (ab50887), IRSp53 (ab15697). CLASP2 antibody was from Santa Cruz Biotechnology (sc-98440). DIXDC1 total antibody was from R&D Systems (AF5599). Phospho-DIXDC1 S592 was developed in collaboration with Antony Wood at Cell Signaling Technologies (CST, Danvers, MA).
Goodwin J.M., Svensson R.U., Lou H.J., Winslow M.M., Turk B.E, & Shaw R.J. (2014). A novel AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Molecular cell, 55(3), 436-450.
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3

Immunoblotting Analysis of IGF/Insulin Signaling

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IGF1R-beta (CST#3027), IGF1R Beta XP (CST#9750), p-IGF1R beta (CST#3918), INSR (CST#3025), IGF2R (CST#14364), AKT (CST#9272), p-AKT (CST#9271); Tubulin #B512 (Sigma); GAPDH (SantaCruz#FL335); Actin (Sigma#A5316), Ubiquilin1 (CST#14526); Anti-FLAG Affinity Gel (Sigma#A2220), INSR (CST#3025), FLAG polyclonal (Sigma# F7425).
Kurlawala Z., Dunaway R., Shah P.P., Gosney J.A., Siskind L.J., Ceresa B.P, & Beverly L.J. (2017). Regulation of Insulin-like Growth Factor Receptors by Ubiquilin1. The Biochemical journal, 474(24), 4105-4118.
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4

Evaluating Enza and LMB Effects

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Enzalutamide (Enza) and leptomycin B (LMB) were obtained from Selleck Chemicals (Houston, TX, USA) and EMD Millipore (Billerica, MA, USA), respectively. Sodium molybdate and cycloheximide were from Sigma (St. Louis, MO, USA). All other chemicals were of analytical grade. Antibodies and their dilutions used for Western blot analysis were FLAG M2 monoclonal (F1804, Sigma; 1:2000), FLAG polyclonal (F7425, Sigma; 1:1000), HSP90 (C45G5, Cell Signaling Technology Danvers, MA; 1:1000), β-actin (13E5, Cell Signaling Technology: 1:1000), androgen receptor (441, Santa Cruz Biotechnology, Dallas, TX, USA: 1:2000), and GAPDH (14C10, Cell Signaling Technology: 1:5000). Horseradish peroxidase-conjugated goat anti-mouse or rabbit IgG were from Santa Cruz Biotechnology. For immunofluorescence studies, FLAG M2 antibody (1:1000) was used in combination with Alexa Fluor 488 Goat Anti-Mouse IgG (H+L) Antibody (A11001, Thermo Fisher Scientific, Waltham, MA, USA; 1:1000) and DAPI (4’, 6-diamidino-2-phenylindole) for nuclear staining.
Uo T., Dvinge H., Sprenger C.C., Bradley R.K., Nelson P.S, & Plymate S.R. (2016). Systematic and functional characterization of novel androgen receptor variants arising from alternative splicing in the ligand-binding domain. Oncogene, 36(10), 1440-1450.
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5

Enzalutamide and Leptomycin B in Cell Signaling

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Enzalutamide (Enza) and leptomycin B were obtained from Selleck Chemicals (Houston, TX, USA) and EMD Millipore (Billerica, MA, USA), respectively. Sodium molybdate and cycloheximide were from Sigma (St Louis, MO, USA). All other chemicals were of analytical grade. Antibodies and their dilutions used for western blot analysis were FLAG M2 monoclonal (F1804; Sigma; 1:2000), FLAG polyclonal (F7425; Sigma; 1:1000), HSP90 (C45G5; Cell Signaling Technology, Danvers, MA, USA; 1:1000), β-actin (13E5; Cell Signaling Technology; 1:1000), androgen receptor (441; Santa Cruz Biotechnology, Dallas, TX, USA; 1:2000) and GAPDH (14C10; Cell Signaling Technology; 1:5000). Horseradish peroxidase-conjugated goat anti-mouse or rabbit IgG were from Santa Cruz Biotechnology. For immunofluorescence studies, FLAG M2 antibody (1:1000) was used in combination with Alexa Fluor 488 Goat Anti-Mouse IgG (H+L) antibody (A11001; Thermo Fisher Scientific, Waltham, MA, USA; 1:1000) and DAPI (4',6-diamidino-2-phenylindole) for nuclear staining.
Uo T., Dvinge H., Sprenger C.C., Bradley R.K., Nelson P.S, & Plymate S.R. (2016). Systematic and functional characterization of novel androgen receptor variants arising from alternative splicing in the ligand-binding domain. Oncogene, 36(10), 1440-1450.
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