Rats were individually kept in metabolic cages for 24 h with free access to water and food. Urine samples were collected and the volume was measured. Blood samples were obtained from tail vein. Urinary and serum creatinine concentrations were measured using a rat creatinine assay kit (Crystal Chem Inc., IL, United States). Creatinine clearance was calculated using a standard formula. Albumin in urine was assessed using a rat microalbumin ELISA (Biocompar, CA, United States). Urinary kidney injury molecule-1 (Kim-1) was measured using a rat kim-1 ELISA kit (Abcam, MA, United States).
Rat kim 1 elisa kit
Manufactured by Abcam
Sourced in United States
The Rat KIM-1 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of Kidney Injury Molecule-1 (KIM-1) levels in rat samples. The kit uses two highly specific antibodies to capture and detect KIM-1 in the sample.
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3 protocols using rat kim 1 elisa kit
1
Renal Function Evaluation in Rats
2
Quantification of Rat Serum Biomarkers
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To measure rat serum HMGB1, IL-18 and urinary KIM-1, we used the Rat HMGB1 ELISA kit (E-EL-R0505, Elabscience, Houston, Tx), Rat IL-18 ELISA kit (ab213909) and Rat KIM-1 ELISA kit (ab223858, Abcam, Cambridge, MA) according to the manufacturer’s instructions. Absorbance levels of the concentrations were measured at 450 nm using a microplate reader (Molecular Devices, Sunnyvale, CA).
3
Kidney Injury Biomarker Quantification
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Urine albumin and creatinine concentrations were determined using kits from Exocell (Philadelphia, PA). Rat KIM-1 ELISA kit was purchased from ABCAM (Cambridge, MA).
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