Cultured nerve cells were treated with 0.25% trypsin (Beyotime). The supernatant was removed, and the nerve cells were suspended in the incubation buffer at a density of 1×106 cells/mL. Nerve cells were then maintained with Annexin V-FITC and propidium iodide (PI) (XiLongScientific) in the dark at room temperature for 15 min. Finally, FACSCalibur (BD Biosciences, San Diego, CA, USA) was performed to measure cell proliferation and apoptosis.
Annexin 5 fitc
Manufactured by Xilong
Annexin V-FITC is a fluorescent-labeled protein that binds to the phospholipid phosphatidylserine. It is commonly used in flow cytometry and microscopy applications to detect and quantify apoptotic cells.
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Lab products found in correlation
2 protocols using annexin 5 fitc
1
Nerve Cell Apoptosis Assay
2
Apoptosis Quantification via Flow Cytometry
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Cell apoptosis was assessed by Flow cytometry (FCM). After washing by PBS, MC3T3-E1 cells were Trypsinized by 0.25% Trypsin (Beyotime, Shanghai, China). MC3T3-E1 cells were centrifuged at 1,000 rpm/min for 1 min, and the supernatant was removed and the MC3T3-E1 cells for assessment were suspended in the incubation buffer at a density of 1×106 cells/ml. And then cells were maintained with Annexin V-FITC and propidium iodide (PI; XiLong Scientific Co., Ltd.) at room temperature for 15 min in the dark. After that, cell apoptosis was assessed by FACSCalibur (BD Biosciences, San Diego, CA, USA).
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