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Biotinylated anti mouse gr1

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Biotinylated anti-mouse Gr1 is a laboratory reagent used for the detection and isolation of mouse Gr1-expressing cells. It is a biotinylated monoclonal antibody that specifically binds to the Gr1 antigen on the surface of mouse cells.

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Lab products found in correlation

B27 supplement, by Thermo Fisher Scientific (2 mentions) Murine egf, by Thermo Fisher Scientific (2 mentions) Biotin selection kit, by STEMCELL (2 mentions) Murine bfgf, by Thermo Fisher Scientific (2 mentions)

2 protocols using biotinylated anti mouse gr1

1

Tumor Cell-MDSC Co-Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor cells (20,000 cells per well) were cultured in low-adhesion 24well plate in DMEM/F12 medium with murine bFGF (20 ng mL−1, Life Technologies), murine EGF (20 ng mL−1, Life Technologies) and B27 supplement (GIBCO). For co-culture, 80,000 Gr1+ MDSCs were added. As indicated, in some instances MDSCs and tumor cells were co-cultured in Boyden chambers, putting MDSCs on insets with 0.4 μm pore size membranes. Co-culture periods were 5 h for RNA expression study, 24 h for FACS of cancer stem cell surface markers, and 6 to 12 d to determine mammosphere forming units. As indicated in figures, specific control groups received Gr1+ cells of TF mice (NN, normal neutrophils) instead of Gr1+ MDSCs. These control cells were isolated from bone marrow with the same magnetic sorting procedure used for MDSCs (Biotinylated anti-mouse Gr1, BD Pharmingen, Biotin Selection Kit, Stem Cell Technologies). Mammospheres were enumerated by manual counting of low magnification images of the cultures (Cellcount). The images in Supplementary Fig. 7a are representative of over 20 images obtained from different experiments. For flow cytometry, mammospheres were dissociated by trypsin-EDTA digestion. Total tumor cell counts were obtained by FACS of the GFP-tagged tumor cells.
Welte T., Kim I.S., Tian L., Gao X., Wang H., Li J., Holdman X.B., Herschkowitz J.I., Pond A., Xie G., Kurley S., Nguyen T., Liao L., Dobrolecki L.E., Mo Q., Edwards D.P., Huang S., Xin L., Xu J., Li Y., Lewis M.T., Wang T., Westbrook T.F., Rosen J.M, & Zhang X.H. (2016). Oncogenic mTOR signaling recruits myeloid-derived suppressor cells to promote tumor initiation. Nature cell biology, 18(6), 632-644.
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2

Tumor Cell-MDSC Co-Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor cells (20,000 cells per well) were cultured in low-adhesion 24well plate in DMEM/F12 medium with murine bFGF (20 ng mL−1, Life Technologies), murine EGF (20 ng mL−1, Life Technologies) and B27 supplement (GIBCO). For co-culture, 80,000 Gr1+ MDSCs were added. As indicated, in some instances MDSCs and tumor cells were co-cultured in Boyden chambers, putting MDSCs on insets with 0.4 μm pore size membranes. Co-culture periods were 5 h for RNA expression study, 24 h for FACS of cancer stem cell surface markers, and 6 to 12 d to determine mammosphere forming units. As indicated in figures, specific control groups received Gr1+ cells of TF mice (NN, normal neutrophils) instead of Gr1+ MDSCs. These control cells were isolated from bone marrow with the same magnetic sorting procedure used for MDSCs (Biotinylated anti-mouse Gr1, BD Pharmingen, Biotin Selection Kit, Stem Cell Technologies). Mammospheres were enumerated by manual counting of low magnification images of the cultures (Cellcount). The images in Supplementary Fig. 7a are representative of over 20 images obtained from different experiments. For flow cytometry, mammospheres were dissociated by trypsin-EDTA digestion. Total tumor cell counts were obtained by FACS of the GFP-tagged tumor cells.
Welte T., Kim I.S., Tian L., Gao X., Wang H., Li J., Holdman X.B., Herschkowitz J.I., Pond A., Xie G., Kurley S., Nguyen T., Liao L., Dobrolecki L.E., Mo Q., Edwards D.P., Huang S., Xin L., Xu J., Li Y., Lewis M.T., Wang T., Westbrook T.F., Rosen J.M, & Zhang X.H. (2016). Oncogenic mTOR signaling recruits myeloid-derived suppressor cells to promote tumor initiation. Nature cell biology, 18(6), 632-644.
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