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3 protocols using iron 2 sulphate

1

Antioxidant Evaluation Protocol

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Chemicals and reagents used such as thiobarbituric acid (TBA), 1,10-phenantroline, gallic acid, Folin–Ciocalteau’s reagent were procured from Sigma-Aldrich, Inc. (St. Louis, MO), trichloroacetic acid (TCA) was sourced from Sigma-Aldrich, Chemie GmbH (Steinheim, Germany), hydrogen peroxide, methanol, acetic acid, hydrochloric acid, sodium carbonate, aluminium chloride, potassium acetate, sodium dodecyl sulphate, iron (II) sulphate, potassium ferricyanide and ferric chloride were sourced from BDH Chemicals Ltd, (Poole, England). Except when stated otherwise, all other chemicals and reagents were of analytical grades and the water was glass distilled.
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2

Biochemical Reagent Sourcing and Preparation

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Chemical reagents such as acetylthiocholine iodide, thiobarbituric acid, sulphanilamide, reduced glutathione, DEPPD, DMSO, DPPH, trichloroacetic acid, and sodium acetate were sourced from Sigma-Aldrich (now Merck KGaA, Darmstadt, Germany). Hydrogen peroxide, methanol, acetic acid, hydrochloric acid, aluminium chloride, potassium acetate, sodium dodecyl sulphate, iron (II) sulphate, manganese chloride, potassium ferrycyanide, and ferric chloride were sourced from BDH Chemicals Ltd., (Poole, England). Except stated otherwise, all other chemicals and reagents were of analytical grades and the water was glass distilled.
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3

Phytochemical Analysis and Antioxidant Assays

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Chemical reagents such as 5,5′-dithio-bis-(2-nitrobenzoic acid (DTNB), 2′,7′-dichlorodihydrofluorescein diacetate, 2-diphenyl-1picrylhydrazyl (2 DPPH), thiobarbituric acid (TBA), GA, TA, porcine pancreatic alpha-amylase and 1,10-phenanthroline were procured from Sigma Aldrich Co. (St Louis, Missouri, USA). Trichloroacetic acid (TCA) was sourced from Sigma Al-drich, Chemie GmbH (Steinheim, Germany). Hydrogen peroxide, methanol, acetic acid, hydrochloric acid, aluminium chloride, potassium acetate, sodium dodecyl sulphate, iron (II) sulphate, potassium ferricyanide and ferric chloride were sourced from BDH Chemicals Ltd., (Poole, England). Ascorbic acid and starch were products of Merck (Darmstadt, Germany). Acarbose was purchased from Glenmark Generics (Europe) pharmaceutical limited. Except stated otherwise, all other chemicals and reagents were of analytical grades and the water was glass distilled.
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