Nk 92

Manufactured by Merck Group

Sourced in United States

NK-92 is a human natural killer cell line derived from a patient with non-Hodgkin's lymphoma. It is a commercially available cell line that can be used for research purposes.

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Lab products found in correlation

Nk 92, by American Type Culture Collection (3 mentions) Adriamycin adr, by Merck Group (1 mentions) 8 pcpt 2 o me camp, by Merck Group (1 mentions) Horse serum, by Merck Group (1 mentions) Recombinant il 2, by Merck Group (1 mentions) Nk 92, by Thermo Fisher Scientific (1 mentions) Farage, by Thermo Fisher Scientific (1 mentions) Sp600125, by Selleck Chemicals (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Farage, by American Type Culture Collection (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions) X vivo 10 media, by Lonza (1 mentions) Khyg 1, by Thermo Fisher Scientific (1 mentions) Human ab serum, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Rpmi 1640, by Merck Group (1 mentions) Mm 1s, by American Type Culture Collection (1 mentions)

3 protocols using nk 92

Immune Cell Activation and Cytotoxicity

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Human immortalized hepatocyte PH5CH8 cells 14 and human hepatoma HuH‐7 cell‐derived RSc cells 15 were cultured as previously described 16. The NK cell line NK‐92 17 was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured according to the manufacturer's instructions. NK‐92 cells were previously reported to induce interferon (IFN)‐γ through cell‐to‐cell contact with influenza A or Sendai virus‐infected macrophages 18. In addition, NK‐92 cells were reported to augment the cytotoxicity against Newcastle disease virus‐infected cells 19.
The DNA‐damaging agent adriamycin (ADR) was purchased from Sigma‐Aldrich (St Louis, MO, USA). The synthetic dsRNA analog poly IC was purchased from Invivogen (San Diego, CA, USA).

Dansako H., Imai H., Ueda Y., Satoh S., Wakita T, & Kato N. (2018). ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell‐mediated innate immune response in human hepatocytes. FEBS Open Bio, 8(3), 361-371.

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Modulation of NHL Cell Lines

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NHL cell lines Toledo, NK-92, RL, and Farage were all purchased from American Type Culture Collection (VA, USA). Toledo, Farage, and RL cells were cultured in RPMI-1640 medium containing 10% FBS (Gibco, CA, USA); NK-92 cells were cultured in Alpha Minimum Essential Medium with ribonucleosides and deoxyribonucleosides free while with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate, 0.2 mM inositol, 0.1 mM 2-mercaptoethanol, 0.02 mM folic acid, 100–200 U/mL recombinant IL-2, 12.5% horse serum (Sigma-Aldrich Co., St Louis, MO, USA), and 12.5% FBS.
Toledo or NK-92 cells were incubated with different concentrations of forskolin (0, 10, 20, 40, 80, or 160 μM; cAMP analog 8-pCPT-2′-OMe-cAMP; Sigma-Aldrich Co) for the indicated times, and 20 μM of SP600125 (Selleck Chemicals, Shanghai, China), an inhibitor of c-Jun N-terminal kinase (JNK) for 24 hours.

Wang H., Lou C, & Ma N. (2019). Forskolin exerts anticancer roles in non-Hodgkin’s lymphomas via regulating Axin/β-catenin signaling pathway. Cancer Management and Research, 11, 1685-1696.

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Myeloma Cell Lines and Patient Samples

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Cell lines MM1S, JJN3, H929, K562, and NK-92 were from American Type Culture Collection (ATCC). KHYG-1 was kindly provided by Dr. Armand Keating (University of Toronto). All cell lines except NK-92 were cultured in RPMI-1640 supplemented with 10% heat-inactivated FBS (Sigma Aldrich), 100 IU/mL of penicillin, and 100 µg/mL of streptomycin. NK-92 cells were cultured in X-VIVO 10 media (Lonza) supplemented with 20% human AB serum (Sigma) and 500 IU/mL of interleukin (IL) 2. KHYG-1 cells were supplemented with 100 IU/mL of IL-2 (Peprotech). Fresh bone marrow (BM) was obtained from patients with MM after informed consent. Mononuclear cells (MNCs) were obtained from BM aspirates (BMAs) after density gradient centrifugation with Ficoll-Paque. CD138⁺ MM cells were isolated by magnetic bead-based positive selection (StemCell Technologies). MNC, CD138^-, and CD138⁺ fractions from BMAs supplied from patients with MM were provided by the Blood Cancer Biobank Ireland.

Daly J., Sarkar S., Natoni A., Stark J.C., Riley N.M., Bertozzi C.R., Carlsten M, & O'Dwyer M.E. (2022). Targeting hypersialylation in multiple myeloma represents a novel approach to enhance NK cell–mediated tumor responses. Blood Advances, 6(11), 3352-3366.

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