Gcms qp2010 se mass spectrometer

Gas chromatography-Electro ionization Mass Spectrometry (GC–EIMS) analysis was performed on a fused silica column (ZB-5-MS, 5% phenyl methyl polysiloxane, 30 m, 0.25 mm i.d., 0.25 µm film thickness; Phenomenex, Torrance, CA, USA) in a GC 2010 chromatograph coupled with GCMS-QP2010 SE mass spectrometer (Shimadzu, Kyoto, Japan) equipped with a quadrupole analyzer. Helium was used as a carrier gas at the flow rate of 1 mL/min. The programmed temperature was set as follows: oven temperature was raised from 100 °C to 325 °C using a ramp of 20 °C/min. The final temperature was maintained for 5 min (end of the analysis). One µL of the sample was injected into the column with a split ratio of 1/10. The MS detector was set as follows: electron impact mode (70 eV) with the ion source temperature set at 200°C, analyzed mass range m/z 40–700. Spectrum was acquired from 3 min (solvent delay) to 16.25 min (end of the run). The identification of the chemical structures was performed by comparison with a library of mass spectra (NIST MS Search 2.0).

Samples were prepared as previously described⁴⁸ ; briefly, a chloroform solution of the total lipid extract (about 1 mg) was dried in a stream of nitrogen at 30 °C, and then refluxed in 0.5 ml methanolic-hydrochloric acid (0.6 N) for 3 h at 80 °C. Fatty acid methyl esters were extracted, in hexane. The hexane extracts were dried under N₂ and then dissolved in chloroform. Fatty acid methyl ester (FAME) composition was determined by gas chromatography with mass spectrometry (GC-MS).
The GC–MS analyses were carried out on a Shimadzu GC 2010 plus gas chromatograph coupled to a Shimadzu GC-MS QP2010 SE mass spectrometer. The chromatographic separations were performed using a SLB-5ms column (30 m × 0.25 mm id, film thickness 0.25 μm). The GC parameters were the following: gas carrier (helium) at the constant flow rate of 0.87 ml/min; the injector (split mode) was at 250 °C; the oven temperature program was 120 °C (5 min) to 180 °C (3 min) at 20 °C/min, to 280 °C (20 min) at 10 °C/min. The MS spectra were obtained in EI mode (70 eV) with the ion source temperature at 200 °C.

Compounds were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The mixture was separated using ZB-5ms column (Phenomenex) and then the single constituents were identified using GC-MS-QP2010 SE mass spectrometer (Shimadzu). The injection volume was 1 µL (split ratio 200:1) and the oven temperature profile was as follows: 50 °C for 2 min followed by an increase of 4 °C min⁻¹ to 280 °C; 280 °C for 2 min; an increase of 40 °C min⁻¹ to 320 °C; 320 °C for 5 min. Helium with the flow of 2 mL min⁻¹ was used as the carrier gas.