Testis tissue was homogenized at 4°C in 1ml QIAzol Lysis Reagent (Qiagen, Hilden, Germany) together with RNase-Free Zirconium Oxide Beads (NextAdvance, Inc., Troy, NY, USA) during 1 min, maximum effect, in a BulletBlender (NextAdvance) and total RNA was prepared according to the QIAzol (Qiagen) protocol. Polyadenylated RNA was isolated with the Dynabeads mRNA direct purification kit (Thermo Fisher Scientific), following the kit protocol. For full-length cDNA Oxford Nanopore sequencing, the cDNA-PCR Sequencing SQK-PCS108 kit (Oxford Nanopore Technologies) was used according to the kit protocol with minor modifications. The finished cDNA library was sequenced on a MinION device using a R9.4 flow cell (Oxford Nanopore Technologies).
Cdna pcr sequencing sqk pcs108 kit
Manufactured by Oxford Nanopore
The cDNA-PCR Sequencing SQK-PCS108 kit is a laboratory equipment product from Oxford Nanopore. The kit is designed for cDNA sequencing using nanopore technology. It provides the necessary reagents and consumables for sample preparation and sequencing on Oxford Nanopore's sequencing platforms.
Automatically generated - may contain errors
Lab products found in correlation
Bullet blender, by Next Advance (2 mentions)
Rnase free zirconium oxide beads, by Next Advance (2 mentions)
Qiazol lysis reagent, by Qiagen (2 mentions)
R9.4 flow cell, by Oxford Nanopore (2 mentions)
Dynabeads mrna direct purification kit, by Thermo Fisher Scientific (2 mentions)
Qiazol, by Qiagen (2 mentions)
2 protocols using cdna pcr sequencing sqk pcs108 kit
1
Testis tissue RNA extraction and sequencing
2
Testis RNA Extraction and Full-length cDNA Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Testis tissue was homogenized at 4°C in 1ml QIAzol Lysis Reagent (Qiagen, Hilden, Germany) together with RNase-Free Zirconium Oxide Beads (NextAdvance, Inc., Troy, NY, USA) during 1 min, maximum effect, in a BulletBlender (NextAdvance) and total RNA was prepared according to the QIAzol (Qiagen) protocol. Polyadenylated RNA was isolated with the Dynabeads mRNA direct purification kit (Thermo Fisher Scientific), following the kit protocol.
For full-length cDNA Oxford Nanopore sequencing, the cDNA-PCR Sequencing SQK-PCS108 kit (Oxford Nanopore Technologies) was used according to the kit protocol with minor modifications. The finished cDNA library was sequenced on a MinION device using a R9.4 flow cell (Oxford Nanopore Technologies).
For full-length cDNA Oxford Nanopore sequencing, the cDNA-PCR Sequencing SQK-PCS108 kit (Oxford Nanopore Technologies) was used according to the kit protocol with minor modifications. The finished cDNA library was sequenced on a MinION device using a R9.4 flow cell (Oxford Nanopore Technologies).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!