The normal human prostate epithelial cells RWPE-1 and PCa cell lines (PC3 and DU145) were purchased from American Tissue Culture Collection (Manassas, VA, USA). All cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) at 37°C and 5% CO2. To construct PTX-resistant PCa cells (PC3-TXR and DU145-TXR), PC3 and DU145 cells were incubated with stepwise increased concentrations of PTX (Selleck, Houston, TX, USA) according to the previous report.22 (link) PC3-TXR and DU145-TXR cells were cultured in normal medium with 10 nM PTX.
Du145
Manufactured by Selleck Chemicals
Sourced in United States
The DU145 is a laboratory instrument used for the quantitative analysis of various chemical and biological samples. It is a spectrophotometer that measures the absorbance or transmittance of light passing through a sample, allowing for the determination of the concentration or presence of specific analytes. The DU145 provides accurate and reliable results for a wide range of applications in research and analytical laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Du145, by American Type Culture Collection (3 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Du145, by National Collection of Authenticated Cell Cultures (2 mentions)
Paclitaxel, by Selleck Chemicals (2 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Rwpe 1, by American Type Culture Collection (1 mentions)
Rpmi 1640 medium, by Wisent (1 mentions)
Gentamicin, by Thermo Fisher Scientific (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Amphotericin b, by Wisent (1 mentions)
Lncap, by Selleck Chemicals (1 mentions)
Htb 81, by American Type Culture Collection (1 mentions)
Olaparib, by Selleck Chemicals (1 mentions)
Crl 174, by American Type Culture Collection (1 mentions)
Hep3b, by American Type Culture Collection (1 mentions)
Ly2109761, by Selleck Chemicals (1 mentions)
Tgf β1, by Selleck Chemicals (1 mentions)
Tgf β1, by R&D Systems (1 mentions)
7 protocols using du145
1
Establishment of PTX-resistant Prostate Cancer Cells
2
Establishing Olaparib-Resistant Prostate Cancer Cell Lines
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Human PC cell lines, LNCaP (RRID:CVCL_0395) and DU145 (RRID:CVCL_0105), were purchased from the American Type Culture Collection (Manassas, VA, USA; ATCC CRL-174 and ATCC HTB- 81, respectively). The LNCaP C4-2B, denoted C4-2B in the study, (RRID:CVCL_4784) cell line was kindly gifted by Dr. Martin Gleave (Vancouver Prostate Centre, BC, Canada). All cell lines were verified by short tandem repeat (STR). Cell lines were cultured in RPMI 1640 medium (Wisent Inc., St-Bruno, QC, Canada; 350-000-EL) supplemented with 10% fetal bovine serum (FBS; Wisent Inc., St-Bruno, QC, Canada; 098–150), 0.5 µg/mL amphotericin B (Wisent Inc., St-Bruno, QC, Canada; 450–105-QL), and 50 µg/mL gentamicin (Life Technologies Inc., Carlsbad, CA, USA; 15710064). All cells were grown in 5% CO2 at 37 °C. OR cells derived from LNCaP, C4-2B and DU145 were obtained by culturing parental cell lines in increasing concentrations (0.5 to 70 µM) of olaparib (Selleckchem, Conshohocken, PA, USA; AZD2281) for 6 months. Doubling time and cell morphology were determined using the IncuCyteTM Live-Cell Imaging System (Essen BioScience, Inc., Ann Arbor, MI, USA).
3
Regulation of OCIAD2 in Cancer Cells
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Hep-3B and Du-145 were obtained from American Type Culture Collection. All cell lines were cultured in DMEM with 10% fetal bovine serum (FBS) and antibiotics. TGFβ1 (R&D Systems, Minneapolis, MN) were applied at concentrations of 5 ng/mL. TGFβR inhibitor LY2109761 were purchased from Selleck Chemicals LLC (Houston, TX), using 2 μM. For the drug treatment, human liver and prostate cancer cell lines, Hep-3B and Du-145 were treated with 5 ng/mL TGFβ1, 2 μM LY2109761, and combination for 24 hours in serum free media, and OCIAD2 mRNA levels were determined by quantitative real-time RT-PCR analysis.
4
CRPC Chemotherapy Resistance in DU145 and PC3
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The focus of this article is to study CRPC chemotherapy resistance, so we selected two hormone resistant cell lines DU145 and PC3 as the research subjects.The human prostate cancer cell lines PC-3 and DU-145 were purchased from the Cell Bank of Shanghai Life Science Institution, Chinese Academy of Sciences and grown in Roswell Park Memorial Institute (RPMI) 1640 media supplemented with 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 1% streptomycin/penicillin. in a humidified atmosphere containing 5%CO2/95% air at 37ºC (Thermo Scientific, Waltham, MA, USA). The docetaxel (51,148, Selleck, Shanghai, China) concentration used to treat PC-3 and DU-145 was 0.1 μmol/L and 5 nmol/L, respectively.We have confirmed that the note of caution stated in Cellosaurus(https://www.cellosaurus.org/CVCL_0035 ) for the PC-3 cell line will not have an impact on our study.
5
Establishing Flavopiridol-Tolerant Prostate Cancer Cell Line
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Human prostate cancer cell line DU145 (ATCC, VA, USA) was routinely cultured in phenol red free RPMI-1640 medium (Cat. No. 11835–063, Gibco) containing 10% of fetal bovine serum (Gibco), 100 U/ml of penicillin and 100 μg/ml of streptomycin (Gibco). To obtain flavopiridol-tolerant DU145 cells, wild type DU145 cells (DU145WT) were maintained in the medium as described above with additional 400 nM flavopiridol hydrochloride (Selleck Chemicals) plus 1 mM sodium pyruvate at final concentration. The flavopiridol-tolerant DU145 cells (DU145FP) were routinely cultured in medium containing 400 nM FP and passaged over a half year to stabilize the features. Cells were cultured in a 37 °C saturated incubator containing 5% of CO2 (BINDER). The medium for DU145FP culture, if not specifically mentioned, always contained 400 nM FP in all experiments.
6
Establishing Paclitaxel-Resistant Prostate Cancer Cell Lines
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Two PCa cell lines (PC3 and DU145) and RWPE-1 normal human prostate epithelial cells were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). All cells were cultured in Roswell Park Memorial Institute 1640 (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (Gibco) and maintained in a humidified atmosphere containing 5% CO2 at 37°C. PR PCa cell lines (PC3-PR and DU145-PR) were established according to a previous report.27 (link) Then PC3-PR and DU145-PR cells were cultured in normal medium with 10 nmol l−1 paclitaxel (Selleck, Houston, TX, USA).
7
Establishment of Paclitaxel-Resistant Prostate Cancer Cell Lines
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Two PCA cell lines (PC3 and DU145) and a normal human prostate epithelial cells RWPE-1 were purchased from National Collection of Authenticated Cell Cultures (Shanghai, China). All cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS, Gibco) and maintained with a humidified atmosphere containing 5% CO 2 at 37 °C. The paclitaxel-resistant (PR) PCA cell lines (PC3-PR and DU145-PR) were established according to the previous report [27] . Then PC3-PR and DU145-PR cells were cultured in normal medium with 10 nM paclitaxel (Selleck, Houston, TX, USA).
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