Anti cd38 pe cy7

The following Abs were used: anti-CD19–ECD, anti-CD27–Pe-Cy5, anti-CD10–FITC, anti-IgD–PE (Beckman Coulter); anti-CD20–APC-H7, anti-CD21–APC (BD Biosciences); anti-CD21–PE, anti-CD38–Pe-Cy7, anti-BR3–FITC (eBioscience), and anti–transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI)–PE (R&D Systems).
Whole blood was washed and stained with cocktails comprising the above Abs, and the RBCs were lysed. At least 80,000 PBMCs were acquired on a nine-color CyAn ADP flow cytometer (Beckman Coulter). The data were analyzed using FlowJo software version 9.2 (TreeStar, FlowJo Africa scheme).
The following B cell subsets were identified based on indicated surface markers: immature/transitional, CD19⁺CD10⁺CD38⁺⁺CD27⁻; naive, CD19⁺CD27⁻CD21⁺; tissue-like memory, CD19⁺CD27⁻CD21⁻; resting memory, CD19⁺CD27⁺CD21⁺; activated mature, CD19⁺CD27⁺CD21⁻; plasmablasts, CD19⁺CD27⁺⁺CD38⁺⁺⁺; unswitched resting memory, CD19⁺CD21⁺CD27⁺IgD⁺; and switched resting memory, CD19⁺CD21⁺CD27⁺IgD⁻. The expressions of BR3 and TACI were also evaluated. The gating strategy is shown in Supplemental Fig. 1.

The effect of calcitriol on the expression of the activation markers CD38 and HLA-DR was evaluated on CD4⁺ and CD8⁺ T cells from Co-HC and HESNs by flow cytometry 72 hours post-infection. Fluorochrome labelled antibodies anti-CD4-APC, anti-CD3-PeCy5, anti-CD8-efluor450, anti-HLA-DR-FITC and anti-CD38-PeCy7 (eBioscience, Santa Clara, CA, USA) were used. Four subpopulations of T cells were identified: HLA-DR⁺CD38⁺, HLA-DR⁺CD38^-, HLA-DR^-CD38⁺, and HLA-DR^-CD38^- (S1 Fig).