Human il4 il10 enzyme immunoassay kit

Total IgE and specific IgE against of cow’s milk proteins (nBos d4; nBos d5; nBos d6; nBos d8) were assessed by enzymatic immunoassay (Phadia 100 ThermoFisher Scientific CAP system, Rodano Milano, Italy). Measurements were expressed as kilounits per liter (kU/L).
The concentrations of IL-4 and IL-10 were measured with a Human IL4/IL10 Enzyme immunoassay kit (Boster Biological Technology, Ltd., Fremont, CA, USA). The IL-5 and IFN- concentrations were measured using the human ELISA assay kit (BioVendor, Brno, Czech Republic). The minimum detection concentrations were 15.6 pg/mL for IL-4, 7.8 pg/mL for IL-5 and IL-10, and 0.78 pg/mL for IFN-γ.

The concentrations of IL-4 and IL-10 were measured with a Human IL4/IL10 Enzyme immunoassay kit (Boster Biological Technology, Ltd., Fremont, CA, USA). The IL-5, IFN-γ and IL-13 concentrations were measured using the human ELISA assay kit (BioVendor). The minimum detection concentrations were 1.5 pg/ml for IL-4, 7.8 pg/ml for IL-5, 0.5 pg/ml for IL-10, 0.99 pg/ml for IFN-γ, and 0.7 pg/ml for IL-13. The ELISAs were conducted according to the manufacturer’s recommendations.

Serum samples were flash frozen and stored at −80°C until further analysis. The concentrations of IL-4 and IL-10 were measured with a Human IL4/IL10 Enzyme immunoassay kit (Boster Biological Technology, Ltd., Fremont, CA, USA) according to the type of stimulation and stimulant. Human IL-5 and IFN-γ ELISA, High Sensitivity (BioVendor, Brno, Czech Republic) were used to detect the IL-5 and IFN-γ serum concentrations. Absorbance was read at 450 nm. The minimum detection concentrations were 15.6 pg/ml for IL-4, 7.8 pg/ml for IL-5 and IL-10, and 0.78 pg/ml for IFN-γ.