Horseradish peroxidase conjugated goat immunoglobulin against rabbit igg

Celecoxib, NS-398, SC-58125, SC-236, nimesulide, and SC-560 were purchased from Cayman Chemical (Ann Arbor, MI, USA). GCDCA, dimethyl sulfoxide (DMSO), L-NAME, MDL-12330A, and indomethacin were from Sigma-Aldrich (St. Louis, MO, USA). COX-1 siRNA, COX-2 siRNA, and scrambled siRNA were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Monoclonal antibody (MAb) against mouse COX-1 and polyclonal antibody against rabbit COX-2 were obtained from Abcam (Cambridge, MA, USA). Mouse MAb against HA tag was purchased from OriGene (Rockville, MD, USA). Synthetic PGE₂ was purchased from Tocris Bioscience (Ellisville, MO, USA). The anti-PSaV capsid MAb and the anti-PSaV VPg polyclonal antibody were previously described (41 (link)). The secondary antibodies used were horseradish peroxidase-conjugated goat immunoglobulin against rabbit IgG (Cell Signaling, Beverly, MA, USA) and mouse IgG (Santa Cruz) and fluorescein isothiocyanate (FITC)-conjugated goat immunoglobulin against rabbit IgG (Jackson Immuno Research Laboratory, West Grove, PA, USA).

NS-398 and SC-560 were purchased from Cayman Chemical (Ann Arbor, MI, USA). Indomethacin and dimethyl sulfoxide (DMSO) were from Sigma-Aldrich (St. Louis, MO, USA). COX-1 and COX-2 siRNAs, which were comprised of three targets for each gene, and scrambled siRNA were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Synthetic PGE₂ was from Tocris Bioscience (Ellisville, MO, USA). Polyclonal antibody against rabbit COX-2 and monoclonal antibody against mouse COX-1 were acquired from Abcam (Cambridge, MA, USA). Mouse monoclonal antibody against FCV capsid protein was from Santa Cruz Biotechnology, Inc. The rabbit polyclonal MNV VPg antibody was a kind gift from Dr. I. Goodfellow, Cambridge University, UK. The secondary antibodies used were horseradish peroxidase-conjugated goat immunoglobulin against rabbit IgG (Cell Signaling, Beverly, MA, USA) and mouse IgG (Santa Cruz), and fluorescein isothiocyanae (FITC)-conjugated goat immunoglobulin against rabbit IgG and mouse IgG (Jackson Immuno Research Laboratory, West Grove, PA, USA).