Ho 1 sirna

Manufactured by Qiagen

Sourced in Italy

The HO-1 siRNA is a laboratory tool designed to facilitate the study of heme oxygenase-1 (HO-1) gene expression. It functions by targeting and silencing the HO-1 gene, allowing researchers to investigate the biological role and function of this important enzyme.

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Lab products found in correlation

Nc sirna, by Qiagen (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) X gal staining kit, by Genlantis (1 mentions)

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SiRNAs

2 protocols using ho 1 sirna

HO-1 Silencing and SP/LPS Treatment

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Cells were transfected with HO-1 siRNA, and Non-Correlated (NC) siRNA (Qiagen, Milan, Italy) as previously reported [25 (link)] In brief, cells (2 X 10⁵) were seeded onto 60-mm dishes in medium without antibiotics; 24 h later, the transfection of siRNAs was carried out with Lipofectamine RNAiMAX (Invitrogen). All transfections were carried out with 20 μM duplex siRNA in medium without FBS and antibiotics. After 24 h, cells were split into 6-well plates to perform further analysis. After 24 h (48 h after transfection), cells were untreated or treated for 24 hours with SP and/or LPS and mRNA analysis were performed by RT-qPCR. Experiments were repeated two times.

Montana G, & Lampiasi N. (2016). Substance P Induces HO-1 Expression in RAW 264.7 Cells Promoting Switch towards M2-Like Macrophages. PLoS ONE, 11(12), e0167420.

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Targeted Overexpression and Knockdown of HO-1 in HMEC Cells

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HMEC cells were plated in antibiotic free media in a 6-well plate at 1 × 10⁵ cells/well a day before transfection. Cells were transfected with Lipofectamine™ 2000 in Opti-MEM I Reduced Serum Medium. Each well was transfected for 5 hr with 4.0 μg of either HO-1 expression plasmid (pcDNA 3.1D/V5-HO-1) or the empty vector (pcDNA 3.1D/V5-His/lacZ) for the targeted over expression of HO-1. The HO-1 expression plasmid was generated by cloning HO-1 cDNA into Hind III and Pme I sites of pcDNA 3.1D/V5-His/lacZ vector (Invitrogen). The HO-1 expressing cassette was released from the vector via restriction digestion and gel-purified. Transfection efficiency was determined using the Genlantis X-Gal Staining™ kit (San Diego, CA) according to the manufacturer's instructions. The percentage of cells with blue stain was examined under the microscope and more than 50% efficiency was obtained (supplementary figure 1). The targeted knockdown of HO-1 was achieved by transfecting cells for 5 hr with HO-1 siRNA (Qiagen, Valencia, CA) at a final concentration of 40 nM. A non-targeting siRNA Scrambled was used as control (Qiagen, Valencia, CA). The over expression and knockdown of the HO-1 gene was assessed by qPCR and Western blot analysis at 3 days after transfection.

Lawal A., Zhang M., Dittmar M., Lulla A, & Araujo J.A. (2015). Heme Oxygenase-1 Protects Endothelial Cells from the Toxicity of Air Pollutant Chemicals. Toxicology and applied pharmacology, 284(3), 281-291.

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