X citeseries 120 q mercury lamp

A Zeiss Axio M2 microscope (Zeiss, Jena, Germany) equipped with an X-cite Series 120 Q mercury lamp (Lumen Dynamics, Mississauga, Canada) was used to obtain brightfield and fluorescence images of the cell populations located on glass slides. Mosaic images with 10% tile overlap of overall glass slides were acquired using 10× objective, microscope’s motorized stage and focus. A single image was formed after multiple images stitching using ZEN software (Zeiss). Both brightfield and fluorescence images were used to determine coordinates of fiducial marks and individual cells (as fluorescent blobs) using the microMS platform as previously reported.^{12 (link)} Geometric information of resulting 2594 points was exported to xeo geometry files compatible with ultrafleXtreme mass spectrometer (Bruker Daltonics, Billerica, MA, USA)

Brightfield and fluorescent images were acquired on an Axio Imager M2 (Zeiss, Jena, Germany) equipped with an AxioCam ICc 5 using a .63× camera adaptor, transmitted light VIS-LED lamp and X-cite Series 120 Q mercury lamp (Lumen Dynamics, Mississauga, Canada). DAPi (ex. 335–383 nm; em. 420–470 nm), GFP (ex. 450–490 nm; em. 500–550) and HE DsRed (ex. 538–562; em. 570–640) dichroic filter cubes were used for imaging. Images were acquired in mosaic mode using a 10× objective with 10 % tile overlap. The resulting tiles were stitched before exporting in TIFF-file format using ZEN 2.0 Pro edition (Zeiss, Jena, Germany) software. Unstained controls were acquired for linear unmixing.