A small‐scale 24 deep well plate (DWP) model was used to assess the performance of individual subclones and the MCB in duplicate in a 10‐day fed‐batch assay. Cells were cultured in 24 deep‐well plates (Axygen, Union City, CA) using proprietary chemically‐defined production medium without MTX. For all conditions, 3 mL working volume per well was used and cultures were cultivated in a humidified incubator (Kuhner AG, Basel, Switzerland) and shaken. The cells were inoculated at 8 × 105 cells/mL and fed on days 3, 6, and 8. Cell density and viability were measured using Vi‐Cell (Beckman Coulter, Fullerton, CA) and concentrations of glucose were measured by a Bioprofile Flex Analyzer (Nova Biomedical, Waltham, MA). pH was not controlled in the 24 DWP cultures. Glucose concentration was maintained between 10 and 12 g/L though supplemental feeding using a 50 g/L stock solution. Samples were collected for titer analysis by HPLC (as described in the Analytical section).
24 deep well plates
Manufactured by Corning
Sourced in United States
The 24 deep-well plates are a laboratory equipment designed to hold and process multiple samples simultaneously. They feature a grid of 24 individual wells, each with a high-volume capacity, suitable for a variety of applications in research and testing environments.
Automatically generated - may contain errors
2 protocols using 24 deep well plates
1
Small-scale Fed-batch Screening of Cell Lines
2
Fungal Spore Cultivation and Sampling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 2
Fresh A. niger NBL-L061 spores were prepared and used for generating sample material by cultivation of the strain in 24 deep well plates (Axygen, Union City, USA) containing 3 ml fermentation medium 2 (15% w/v maltose, 6% w/v bacto-soytone, 1.5% w/v (NH4)2SO4, 0.1% w/v NaH2PO4.H2O, 0.1% w/v MgSO4.7H2O, 0.1% w/v L-arginine, 8‰ w/v Tween-80, 2‰ w/v Basildon, 2% w/v MES, pH 5.1). The 24 deep well plates were covered with a Breathseal (Greiner bio-one, Frickenhausen, Germany) and a lid. After 6 days of growth at 34° C., 550 rpm and 80% humidity in a Microton incubator shaker (Infors AG, Bottmingen, Switzerland) 1.5 mL samples were taken, the mycelium was separated from the supernatant by centrifugation for 30 min at 4000 g and the supernatants were stored at −20° C. until further analyses.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!