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5 dithiobis 2 nitrobenzoic acid

Manufactured by Merck Group
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Sourced in Japan, United States, United Kingdom, India

5-dithiobis-2-nitrobenzoic acid is a chemical compound commonly used as a reagent in biochemical and analytical applications. It is a yellow crystalline solid that is soluble in water and organic solvents. The compound is known for its ability to react with sulfhydryl groups, making it useful for the detection and quantification of free thiol groups in proteins and other biological molecules.

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Lab products found in correlation

Butyrylthiocholine iodide, by Merck Group (2 mentions) Acetylthiocholine iodide, by Merck Group (2 mentions) Sodium chloride (nacl), by Fujifilm (1 mentions) Galantamine, by Merck Group (1 mentions) Magnesium chloride, by Fujifilm (1 mentions) Triton x 100, by Fujifilm (1 mentions) Tris hcl buffer, by Fujifilm (1 mentions) Donepezil, by Merck Group (1 mentions) Xylenol orange, by Merck Group (1 mentions) 2 4 dinitrophenylhydrazine, by Merck Group (1 mentions) 2 2 dithiodipyridine, by Merck Group (1 mentions) 4 amino tempo, by Merck Group (1 mentions) 2 4 6 tripyridyl s triazine, by Merck Group (1 mentions) O phthalaldehyde, by Merck Group (1 mentions) Muscarine chloride, by Merck Group (1 mentions) Zinc chloride, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Trichloroacetic acid, by Merck Group (1 mentions) Peroxidase, by Merck Group (1 mentions) Cholesterol standard, by Merck Group (1 mentions)

5 protocols using 5 dithiobis 2 nitrobenzoic acid

1

Acetylcholinesterase Inhibition Assay

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Chemicals that were used in this study are analytical grade. Acetylthiocholine iodide (ATCI), butyrylthiocholine iodide (BTCI), donepezil, galantamine, 5’dithio-bis-(2-nitro) benzoic acid, and SK were purchased from Sigma-Aldrich (Japan). Tris-HCl buffer, sodium chloride, magnesium chloride, and Triton X-100 were collected from Wako Pure Chemical Company Ltd. (Japan). Solvents (methanol, chloroform, etc.) were used in this experiment were analytical grade also.
Biswas K., Azad A.K., Sultana T., Khan F., Hossain S., Alam S., Chowdhary R, & Khatun Y. (2017). Assessment of in-vitro cholinesterase inhibitory and thrombolytic potential of bark and seed extracts of Tamarindus indica (L.) relevant to the treatment of Alzheimer’s disease and clotting disorders. Journal of Intercultural Ethnopharmacology, 6(1), 115-120.
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2

Spin Label and Redox Assay Reagents

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The following chemicals were purchased from Sigma Chemical Co. (St. Louis, MO, USA): 4-iodoacetamide-TEMPO (iodoacetamide spin label; ISL), 4-Amino-TEMPO (tempamine), 2, 4, 6-tripyridyl-s-triazine (TPTZ), o-phthalaldehyde (OPA), xylenol orange, 5-dithiobis-2-nitrobenzoic acid (DTNB), acetylthiocholine iodide, 2, 4-dinitrophenylhydrazine (DNPH), and 2, 2′-dithiodipyridine. Bis-(2, 2, 5, 5-tetramethyl-3-imidazoline-1-oxyl-4-yl) disulfide biradical (•RSSR•) was obtained from Enzo Life Sciences, Inc. (New York, USA). All other reagents of analytical purity were obtained from POCH S.A. (Gliwice, Poland).
Gwozdzinski L., Pieniazek A., Bernasinska-Slomczewska J., Hikisz P, & Gwozdzinski K. (2021). Alterations in the Plasma and Red Blood Cell Properties in Patients with Varicose Vein: A Pilot Study. Cardiology Research and Practice, 2021, 5569961.
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3

Muscarinic Receptor Activation Protocol

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Muscarine chloride, zinc chloride, dithiothreitol, 5-dithio-bis(2-nitrobenzoic acid), and methanethiosulfonates were all purchased from Sigma-Aldrich, UK. For high potassium (25 mM K) or acidic pH (pH 6.4) experimentation, the extracellular solution was adjusted accordingly. Muscarine chloride was added directly to extracellular solution at a concentration of 0.1 μM prior to use.
Cousin M.A., Veale E.L., Dsouza N.R., Tripathi S., Holden R.G., Arelin M., Beek G., Bekheirnia M.R., Beygo J., Bhambhani V., Bialer M., Bigoni S., Boelman C., Carmichael J., Courtin T., Cogne B., Dabaj I., Doummar D., Fazilleau L., Ferlini A., Gavrilova R.H., Graham JM J.r., Haack T.B., Juusola J., Kant S.G., Kayani S., Keren B., Ketteler P., Klöckner C., Koopmann T.T., Kruisselbrink T.M., Kuechler A., Lambert L., Latypova X., Lebel R.R., Leduc M.S., Leonardi E., Lewis A.M., Liew W., Machol K., Mardini S., McWalter K., Mignot C., McLaughlin J., Murgia A., Narayanan V., Nava C., Neuser S., Nizon M., Ognibene D., Park J., Platzer K., Poirsier C., Radtke M., Ramsey K., Runke C.K., Guillen Sacoto M.J., Scaglia F., Shinawi M., Spranger S., Tan E.S., Taylor J., Trentesaux A.S., Vairo F., Willaert R., Zadeh N., Urrutia R., Babovic-Vuksanovic D., Zimmermann M.T., Mathie A, & Klee E.W. (2022). Gain and loss of TASK3 channel function and its regulation by novel variation cause KCNK9 imprinting syndrome. Genome Medicine, 14, 62.
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4

Spectrophotometric Quantification of GSH

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A method described by Salbitani et al. (2017) was used to estimate the GSH content [43 (link)]. We mixed 100 µL of tissue homogenate with 10% trichloroacetic acid (Sigma-Aldrich, Bangalore, India) and poured it into a test tube. Then, 5′-dithiobis (2-nitrobenzoic acid) (Sigma-Aldrich, Bangalore, India) and 3.0 mL of phosphate buffer (pH 8.4) were added to the supernatant after centrifugation at 5000 rpm for 10 min. A spectroscopy measurement at 412 nm was performed within 10 min of mixing the mixture. Protein concentrations of GSH were expressed as µg/mg.
Alkholifi F.K., Devi S., Yusufoglu H.S, & Alam A. (2023). The Cardioprotective Effect of Corosolic Acid in the Diabetic Rats: A Possible Mechanism of the PPAR-γ Pathway. Molecules, 28(3), 929.
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5

Assessing Biochemical Markers in Rat Serum

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Monosodium L-glutamate (MSG), 5, 5-dithiobis-2-nitrobenzoic acid, butyrylthiocholine iodide (BTCI), glucose oxidase, peroxidase, cholesterol standard, 4-aminophenazone and thiobarbituric acid were purchased from Sigma-Aldrich, Bangalore. For the quantitative analysis of rat serum insulin, the ELISA kit was purchased from EMD Millipore, USA. All other reagents used were of analytical grade and obtained locally.
Madhavadas S., Subramanian S, & Kutty B.M. (2017). Environmental enrichment improved cognitive deficits more in peri-adolescent than in adult rats after postnatal monosodium glutamate treatment. Physiology international, 104(4).
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