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Mirvana mirna assays

Manufactured by Thermo Fisher Scientific
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The MirVana miRNA assays are a set of tools designed for the detection and quantification of microRNA (miRNA) molecules. These assays provide a reliable and sensitive method for measuring the expression levels of miRNAs, which are small, non-coding RNA molecules that play a crucial role in gene regulation.

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Lab products found in correlation

Mirvana mirna extraction kit, by Thermo Fisher Scientific (2 mentions) Mirvana mirna mimic, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)

2 protocols using mirvana mirna assays

1

Transient miRNA Overexpression Assay

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Pre-miR miRNA mimic (Ambion) or mirVana miRNA mimic (Ambion) were transfected using Lipofectamine RNAiMAX (Invitrogen; standard forward transfection) with a final miRNA concentration of 25 nM. Total RNA and miRNA were isolated using mirVana miRNA extraction kit (Ambion). miRNA stem loop RT-qPCR was performed using mirVana miRNA assays (Ambion) according to manufacturer’s instructions, normalized to U6-snRNA (as internal small RNA control).
Hydbring P., Wang Y., Fassl A., Li X., Matia V., Otto T., Choi Y.J., Sweeney K.E., Suski J.M., Yin H., Bogorad R.L., Goel S., Yuzugullu H., Kauffman K., Yang J., Jin C., Li Y., Floris D., Swanson R., Ng K., Sicinska E., Anders L., Zhao J.J., Polyak K., Anderson D.G., Li C, & Sicinski P. (2017). Cell Cycle-Targeting MicroRNAs as Therapeutic Tools Against Refractory Cancers. Cancer cell, 31(4), 576-590.e8.
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2

Quantifying miRNA Expression in Organs and Tumors

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Organs and tumors were dissected from animals treated with nanoparticles containing control miRNA (Ambion, Negative control #1), miR-193a-3p, or miR-214-5p, at the end of treatment period (20–34 days). RNA was extracted using mirVana miRNA extraction kit (Ambion). miRNA stem loop RT-qPCR was performed using mirVana miRNA assays (Ambion) according to manufacturer’s instruction, with U6-snRNA as internal small RNA control. In each organ or tumor, we determined the delta-Ct values relative to U6-snRNA. Delta-Ct values were then transformed into percentage expression relative to U6-snRNA. For example, an organ displaying a Ct value of 20 for miR-193a-3p and a Ct value of 18 for U6-snRNA has the delta-Ct value of 2, which is transformed into a percentage expression relative to U6-snRNA of 25 %.
Hydbring P., Wang Y., Fassl A., Li X., Matia V., Otto T., Choi Y.J., Sweeney K.E., Suski J.M., Yin H., Bogorad R.L., Goel S., Yuzugullu H., Kauffman K., Yang J., Jin C., Li Y., Floris D., Swanson R., Ng K., Sicinska E., Anders L., Zhao J.J., Polyak K., Anderson D.G., Li C, & Sicinski P. (2017). Cell Cycle-Targeting MicroRNAs as Therapeutic Tools Against Refractory Cancers. Cancer cell, 31(4), 576-590.e8.
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