Thermal difference spectra were collected on a dual beam Evolution 260 Bio UV-Vis spectrophotometer (Thermo Scientific). RNA were diluted in TEK buffer to a final concentration of 1.5 μM and the spectra measured at 20°C was subtracted from the spectra measured at 90°C for each sample. Difference spectra were obtained in triplicate and normalized to the maximal absorbance value for a given spectrum.
Evolution 260 bio uv vis spectrophotometer
Manufactured by Thermo Fisher Scientific
The Evolution 260 Bio UV-Vis spectrophotometer is a laboratory instrument used to measure the absorption of light by a sample across the ultraviolet and visible wavelength range. It is designed for basic absorbance measurements and quantitative analysis.
Automatically generated - may contain errors
3 protocols using evolution 260 bio uv vis spectrophotometer
1
Thermal Difference Spectra of RNA
2
Characterization of Quantum Dot Assemblies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TEM characterization was carried out using a Thermo Fisher FEI Tecnai Spirit Transmission Electron Microscopy operating at 120 kV. For QDs with organic ligands, 10 μl of QDs (50 μg/ml) was drop casted on 400-mesh carbon film square grids (Thermo Fisher Scientific, catalog number: 5024891). For DNA origami and QD/QR-origami assemblies, 10 μl of wireframe DNA origami objects with or without attached QDs/QRs (5 nM) was adsorbed on glow-discharged 400-mesh carbon film square grids and stained by 2% aqueous uranyl formate solution containing 25 mM NaOH.
AFM measurements were performed under air condition in either on an Icon Atomic Force Microscope (Bruker) in ScanAsyst mode using a ScanAsyst-Air silicon tip on nitride lever (tip radius = 2 nm, k = 0.4 N/m, fo = 70 kHz; Bruker) or on an Asylum Research Jupiter XR AFM (Oxford Instruments) in tapping mode using an ARROW-UHF ultrahigh-frequency probe (tip radius < 10 nm, fo = 2000 kHz; NanoWord).
Absorbance spectra were measured using an Evolution 260 Bio UV-vis spectrophotometer (Thermo Fisher Scientific), and steady-state emission spectra (λex = 450 nm) were measured using a multimode microplate reader (Tecan Spark). Quantum yields of QDs/QRs were determined using the relative quantum yield determination method with rhodamine 101 in spectroscopic-grade ethanol as standard (λex = 480 nm, Φs = 0.92) (79 (link)).
AFM measurements were performed under air condition in either on an Icon Atomic Force Microscope (Bruker) in ScanAsyst mode using a ScanAsyst-Air silicon tip on nitride lever (tip radius = 2 nm, k = 0.4 N/m, fo = 70 kHz; Bruker) or on an Asylum Research Jupiter XR AFM (Oxford Instruments) in tapping mode using an ARROW-UHF ultrahigh-frequency probe (tip radius < 10 nm, fo = 2000 kHz; NanoWord).
Absorbance spectra were measured using an Evolution 260 Bio UV-vis spectrophotometer (Thermo Fisher Scientific), and steady-state emission spectra (λex = 450 nm) were measured using a multimode microplate reader (Tecan Spark). Quantum yields of QDs/QRs were determined using the relative quantum yield determination method with rhodamine 101 in spectroscopic-grade ethanol as standard (λex = 480 nm, Φs = 0.92) (79 (link)).
3
RNA Thermal Denaturation Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA was measured at a concentration of 5 µM in TEK buffer. UV-Vis spectra were collected using a temperature-controlled Evolution 260 Bio UV-Vis spectrophotometer (Thermo Scientific) from 340 to 220 nm at 20°C and 90°C. The 20°C spectrum was then subtracted from the 90° spectrum and then normalized to the wavelength with the highest difference.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!