Expression of fluorescent marker genes (i.e., ZsGreen, DsRed, and tdTomato) in plant tissues was observed using a Stemi SVII stereomicroscope equipped with an HBO illuminator (Zeiss, Thornwood, NY, USA), a FITC filter set (λexcitation = 480 nm, and λemission = 515 nm; Chroma Technology, Bellows Falls, VT, USA), and a DsRED filter (λexcitation = 545/25 nm, dichroic 565LP, λemission = 605/70 nm; Chroma Technology). Expression of ZsGreen in isolated embryos was observed under a Zeiss Axioskop 2 plus fluorescence microscope equipped with an 89 North® PhotoFluor LM-75 illumination system (Chroma Technology) and a FITC filter set. Images were taken using an AxioCam camera (Carl Zeiss, Oberkochen, Germany) and the AxioVision LE64 software.
89 north photofluor lm 75 illumination system
Manufactured by Chroma Technology
The 89 North® PhotoFluor LM-75 illumination system is a compact, high-performance LED light source designed for fluorescence microscopy applications. It provides stable, uniform illumination across a wide range of excitation wavelengths.
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Lab products found in correlation
Axioskop 2 plus, by Zeiss (4 mentions)
Axiocam camera, by Zeiss (2 mentions)
Hbo illuminator, by Zeiss (2 mentions)
Axiovision le64, by Zeiss (2 mentions)
Ds red filter, by Chroma Technology (1 mentions)
Fitc filter set, by Chroma Technology (1 mentions)
Fitc lter set, by Chroma Technology (1 mentions)
2 protocols using 89 north photofluor lm 75 illumination system
1
Fluorescent Marker Visualization in Plants
2
Fluorescent Marker Imaging in Plants
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Expression of uorescent marker genes (i.e., ZsGreen, DsRed, and tdTomato) in plant tissues was observed using a Stemi SVII stereomicroscope equipped with an HBO illuminator (Zeiss, Thornwood, NY, USA), a FITC lter set (λ excitation = 480 nm, and λ emission = 515 nm; Chroma Technology, Bellows Falls, VT, USA), and a DsRED lter (λ excitation = 545/25 nm, dichroic 565LP, λ emission = 605/70 nm; Chroma Technology). Expression of ZsGreen in isolated embryos was observed under a Zeiss Axioskop 2 plus uorescence microscope equipped with an 89 North ® PhotoFluor LM-75 illumination system (Chroma Technology) and a FITC lter set. Images were taken using an AxioCam camera (Carl Zeiss, Oberkochen,
Germany) and the AxioVision LE64 software.
Germany) and the AxioVision LE64 software.
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