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89 north photofluor lm 75 illumination system

Manufactured by Chroma Technology

The 89 North® PhotoFluor LM-75 illumination system is a compact, high-performance LED light source designed for fluorescence microscopy applications. It provides stable, uniform illumination across a wide range of excitation wavelengths.

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2 protocols using 89 north photofluor lm 75 illumination system

1

Fluorescent Marker Visualization in Plants

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Expression of fluorescent marker genes (i.e., ZsGreen, DsRed, and tdTomato) in plant tissues was observed using a Stemi SVII stereomicroscope equipped with an HBO illuminator (Zeiss, Thornwood, NY, USA), a FITC filter setexcitation = 480 nm, and λemission = 515 nm; Chroma Technology, Bellows Falls, VT, USA), and a DsRED filterexcitation = 545/25 nm, dichroic 565LP, λemission = 605/70 nm; Chroma Technology). Expression of ZsGreen in isolated embryos was observed under a Zeiss Axioskop 2 plus fluorescence microscope equipped with an 89 North® PhotoFluor LM-75 illumination system (Chroma Technology) and a FITC filter set. Images were taken using an AxioCam camera (Carl Zeiss, Oberkochen, Germany) and the AxioVision LE64 software.
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2

Fluorescent Marker Imaging in Plants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Expression of uorescent marker genes (i.e., ZsGreen, DsRed, and tdTomato) in plant tissues was observed using a Stemi SVII stereomicroscope equipped with an HBO illuminator (Zeiss, Thornwood, NY, USA), a FITC lter set (λ excitation = 480 nm, and λ emission = 515 nm; Chroma Technology, Bellows Falls, VT, USA), and a DsRED lter (λ excitation = 545/25 nm, dichroic 565LP, λ emission = 605/70 nm; Chroma Technology). Expression of ZsGreen in isolated embryos was observed under a Zeiss Axioskop 2 plus uorescence microscope equipped with an 89 North ® PhotoFluor LM-75 illumination system (Chroma Technology) and a FITC lter set. Images were taken using an AxioCam camera (Carl Zeiss, Oberkochen,
Germany) and the AxioVision LE64 software.
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