Pd98095

Manufactured by Selleck Chemicals

PD98095 is a chemical compound used in laboratory research. It serves as a selective inhibitor for the extracellular signal-regulated kinase (ERK) pathway. This product is intended for use in scientific investigations and experiments.

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Lab products found in correlation

Fetal bovine serum (fbs), by Sartorius (1 mentions) Sp600125, by MedChemExpress (1 mentions) Dulbecco modified eagle medium (dmem), by Procell (1 mentions) Sb203580, by MedChemExpress (1 mentions) Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Ly317615, by Selleck Chemicals (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) A 438079, by Selleck Chemicals (1 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Sybr premix ex taqtm tli rnaseh plus, by Takara Bio (1 mentions)

2 protocols using pd98095

Investigating Inflammation Modulation by EP

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HBZY-1 cells were cultured in DMEM (Procell Life Science & Technology Co., Ltd.) containing 10% FBS (Biological Industries) at 37°C in a humidified incubator with 5% CO₂. Cells (3×10³/well) were seeded in a 96-well plate and cultured for 24 h. Cells were pretreated with EP (100 µg/ml), PD98095 (ERK inhibitor; 50 µmol/l; Selleck Chemicals), SP600125 (JNK inhibitor; 10 µmol/l; MedChemExpress) or SB203580 (p38 inhibitor; 10 µmol/l; MedChemExpress) for 1 h and then stimulated with LPS (1 µg/ml) for 24 h, all at 37°C.

Shi Q., Lang W., Wang S., Li G., Bai X., Yan X, & Zhang H. (2020). Echinacea polysaccharide attenuates lipopolysaccharide-induced acute kidney injury via inhibiting inflammation, oxidative stress and the MAPK signaling pathway. International Journal of Molecular Medicine, 47(1), 243-255.

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Modulation of Cardiac Cell Signaling

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The P2X7R inhibitor (A438079), PKC inhibitor (LY317615), and ERK-specific inhibitor (PD98095) were purchased from Selleck (Shanghai, China). A RevertAid First Strand cDNA Synthesis Kit was obtained from Thermo Fisher Scientific (Waltham, MA, United States). SYBR Premix Ex Taq^TM (TliRnaseH Plus) was obtained from Takara (Dalian, China). The primary anti-P2X7R antibody (#APR-004) was obtained from Alomone Labs (Israel). Anti-collagen 1 (anti-COL-1, ab34710), anti-matrix metalloproteinase-9 (anti-MMP9, ab38898), and anti-heavy chain cardiac myosin (ab185967) antibodies were obtained from Abcam (Cambridge, United Kingdom). Moreover, antibodies against TGFβ (#3711), GAPDH (#5174), Caspase-3 (#9662), Bcl2 (#2870), phospho-PKCβII (Thr638/641) (#9375), Bax (#5023), and Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204; #4370) were obtained from Cell Signaling Technology (Danvers, MA, United States).

Huang S., Wang W., Li L., Wang T., Zhao Y., Lin Y., Huang W., Wang Y, & Huang Z. (2021). P2X7 Receptor Deficiency Ameliorates STZ-induced Cardiac Damage and Remodeling Through PKCβ and ERK. Frontiers in Cell and Developmental Biology, 9, 692028.

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