Male balb c mice

Male BALB/c mice (6 weeks, 18–22 g) were obtained from GemPharmatech (Jiangsu, China). Mice were randomly assigned to 4 groups with 10 mice in each group. O157:H7 (5 × 10⁶ CFU/mouse) was administered intraperitoneally to the mice. Thirty minutes later, the mice were injected intraperitoneally with AP-64, Gm94, or LL-37 at the dose of 500 μg/kg. An equivalent volume of PBS was used as a control. All animal experiments were approved by the West China Hospital of Sichuan University Biomedical Ethics Committee (ethic approval document: 2020166A), and all experiments conformed to all relevant regulator standards.

8 week-old male db/db mice, their db/m littermates, and male BALB/c mice were purchased from the GemPharmatech Co., Ltd., (Nanjing China). Animals were maintained in a controlled environment (12 h light/dark cycle at 23°C) with free access to food and water. The experiments were performed following the National Institutes of Health guidelines and with approval from the Animal Care and Use Committee of Wenzhou Medical University, China.
For the DN model, db/db mice were intraperitoneally (i.p.) injected with FGF1^ΔHBS at 0.5 mg/kg body weight every other day for 8 weeks while db/m and db/db mice were received 0.9% normal saline as controls. Blood glucose levels were measured using a blood glucose monitor (Roche).
For the adriamycin-induced nephropathy (AN) model, mice were injected with a single dose of ADR (11 mg/kg) through the tail vein. FGF1^ΔHBS (0.5 mg/kg body weight) or normal saline was administered i.p. every other day starting one week before ADR injection and lasting for 5 weeks. Metabolic cages (TSE Systems, MO) were chosen to collect mice urine for 24 h.

CVB3 virus (Nancy strain) was maintained in our lab and passaged in HeLa cells. HeLa cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS at 37 °C in 5% CO₂ [21 (link)].
Male BALB/c mice aged 4–5 weeks were purchased from Gempharmatech Co., Ltd. (Nanjing, China), and housed in the SPF condition. All animal experiments were performed with the approvement of the Laboratory Animal Center and the Ethics Committee of Soochow University.