Irdye 800cw 926 32212

The antibodies used were as follows: anti-Banf1 N-terminus (SAB1409950, Sigma-Aldrich and ab88464, Abcam, 1:1000 for WB and 1:500 for IF), anti-Banf1 C-terminus (PRS40170604, Sigma-Aldrich, 1:1000 for WB and 1:500 for IF), PARP1 (9532, Cell Signalling Technology 1:1000 for WB and 1:500 for IF), PARP1 (ab191217, Abcam Fig. 2e 1:500 for IF), anti-Emerin (5430, Cell Signalling Technology, 1:500 for IF), anti-Flag M2 Antibody (F3165, Sigma-Aldrich, 1:1000 for WB and 1:300 for IF), anti-PAR (ab14459, Abcam, 1:1000 for WB), anti-γ-Tubulin (T6557, Sigma-Aldrich, 1:2000 for WB), anti-H3 (4499, Cell Signalling Technology, 1:2000 for WB), anti-H4 (2935 Cell Signalling Technology 1:1000 for WB), anti-SP1 (9389, Cell Signalling Technology, 1:1000 for WB), anti-EGFR (sc-03, Santa-cruz, 1:500 for WB), anti-LC3B (2775, Cell Signalling Technologies, 1:1000 for WB), anti-phospho-p53 ser15 (2984, Cell Signalling Technology, 1:1000 for WB), anti-β-actin (612656, BD Biosciences, 1:2000 for WB). Fluorescent secondary antibodies used were: Donkey anti-Mouse 800 nm (LiCor; IRDye 800CW 926-32212, 1:5000 for WB), Donkey anti-Rabbit (LiCor; IRDye 680LT 926-28023, 1:5000 for WB) and Alexa Fluor 488 (Cat# A32766, Molecular Probes, 1:200 for IF) and 594 (Cat# A32754, Molecular Probes, 1:200 for IF).

The antibodies used were as follows: anti-Flag M2 (F3165, Sigma-Aldrich at 1:500 for immunofluorescence (IF) and 1:1,000 for western blotting (WB), anti-Emerin (5430S, Cell Signalling Technology at 1:500 for IF), anti-Actin Ab-5 (612,656, Bioscience International at 1:3,000 for WB). For IF, secondary antibodies; Alexa Fluor 488 (A32766, Molecular Probes, 1:300) and 594 (A32754, Molecular Probes, 1:300). Secondary antibodies used for WB were Donkey anti-Mouse 800 nm (IRDye 800CW 926-32212, LiCor, 1:5,000), Donkey anti-Rabbit (IRDye 680LT 926-28023, LiCor, 1:5,000).