Proteins from tissues or cultured cells were extracted, transferred onto
PVD-membranes, hybridized overnight with appropriate primary antibodies
(
PPARGC1A - sc-13067 and
ab-54481,
DIO2-sc-98716,
BCL-xL- ab32124,
PINK1-ab23707,
LC3B-ab51520,
p62/SQSTM1-ab56416,
BAX-ab32503 and ab-53154,
β-actin-sc-47778) obtained from Santa Cruz Biotechnology, CA (sc), or
Abcam, Cambridge, UK (ab) and visualized using the Gel Doc XR+ System
(Biorad, Lab. Inc. Life Science, USA), according to manufacturer’s
protocol.
Yu G., Tzouvelekis A., Wang R., Herazo-Maya J.D., Ibarra G.H., Srivastava A., de Castro J.P., DeIuliis G., Ahangari F., Woolard T., Aurelien N., e Drigo R.A., Gan Y., Graham M., Liu X., Homer R.J., Scanlan T.S., Mannam P., Lee P.J., Herzog E.L., Bianco A.C, & Kaminski N. (2017). Thyroid hormone inhibits lung fibrosis in mice by improving epithelial mitochondrial function. Nature medicine, 24(1), 39-49.