Sk lu 1

A549 cells were obtained from the American Type Culture Collection and SK-LU-1, HCC-827, and A427 cells were purchased from the Korean Cell Line Bank. NCI-H2009 cells were kindly provided from Dr. Jong Ho Park (Korea Institute of Radiological and Medical Sciences, Korea). A549, SK-LU-1, A427, and NCI-H2009 cells were cultured in Dulbecco's modified Eagle's medium (Life technologies, Grand Island, NY) and HCC-827 cells were cultured in RPMI-1640 medium (Life technologies, Grand Island, NY). All media was supplemented with 5% FBS, 100 unit/ml penicillin, and 0.1 mg/ml streptomycin at 37°C. Stable cell lines expressing only vector or kinase-dead (K297R) Src were generated by transfection of A549 pLNCX or pLNCX-kinase-dead (K297R) Src, which was kindly provided by Dr. Youn Soo Kim (Inje University, Korea), into A549 cells by the calcium phosphate method and selection with 1 mg/mL neomycin.

The human lung cancer cell lines H358 and SK-LU-1 were obtained from the American Type Culture Collection (ATCC). The human embryonic kidney (HEK) cell line HEK293T and the mouse lung LLC cell line were obtained from the Shanghai Institute of Cell Biology, Chinese Academy of Sciences (Shanghai, China). Cells were certified by short tandem repeat (STR) analysis and regularly checked for mycoplasma contamination. Ras-less MEF cell lines overexpressing different KRAS mutations were obtained from the NIH RAS Initiative and cultured as indicated in https://www.cancer.gov/research/key-initiatives/ras/ras-central/blog/2017/rasless-mefs-drug-screens (33 ). H358 cells were maintained in RPMI 1640 medium (C11875500BT, Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (10099-141, Gibco, Thermo Fisher Scientific), and SK-LU-1, LLC, and HEK293T cells were maintained in high-glucose (4.5 g/L) DMEM (C11995500BT, Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco, Thermo Fisher Scientific). All cells were incubated in 5% CO₂ at 37°C in a humidified atmosphere.

Calu‐6, SW1573, SW480, T84, SK‐LU‐1, DLD‐1, HCT8, NCI‐H2122, NCI‐H1299, and NCI‐H460 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). LoVo, NCI‐H358, LS174T, and Calu‐1 cells were purchased from Cell Bank of Shanghai Institutes for Biological Sciences, Chinese Academy of Science (Shanghai, China). A549, HCC2998, and NCI‐H23 were kindly provided by National Institute of Biological Sciences (Beijing, China). Calu‐6, LS174T, LoVo, SW1573, T84, and SK‐LU‐1 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Gaithersburg, MD, USA). A549, DLD‐1, HCT8, SW480, HCC2998, NCI‐H2122, NCI‐H23, NCI‐H1299, NCI‐H460, and NCI‐H358 cells were maintained in RPMI‐1640 (Gibco). Calu‐1 cells were maintained in McCoy’s 5A medium (Gibco). All growth media were supplemented with 10% FBS (Thermo Scientific, Waltham, MA, USA), 100 units·mL⁻¹ penicillin (Gibco), and 0.1 mg·mL⁻¹ streptomycin (Gibco). Cell lines were reinstated from frozen stocks and passaged no more than thirty times.

The lung cancer cell lines A549 and H1975 were obtained from the Cell Bank of Shanghai Institutes for Biological Sciences (Shanghai, China). SK-LU-1 cell line was purchased from Cell Resource Center of Chinese Academy of Medical Sciences (Beijing, China). H1975 cells were cultured in RPMI 1640 medium (Gibco, United States), and A549 cells were cultured in F12K medium (Gibco, United States). SK-LU-1 cells were maintained in MEM medium (Gibco, United States). All culture media were supplemented with 10% fetal bovine serum (FBS) at 37°C in an incubator with 5% CO₂. MCM5-Flag, MCM5, and HDAC1 expression plasmids were purchased from Sino Biological (Beijing, China).

NCI–H292 (CRL-1848) was obtained from ATCC (Manassas, VA, USA). A549 (BNCC337696) was obtained from BeNa Culture Collection (Beijing, China). SK-LU-1 (SNL-492) and NCI–H358 (SNL-392) were obtained from Sunncell Biotech (Wuhan, China). NCI-H1299 (CL-0165) and H1975 (CL-0298) cells were obtained from Procell Life Science&Technology (Wuhan, China). HEK293FT (R70007) cells were from Thermo Fisher Scientific (Waltham, MA, USA).
A549, HEK293FT, SK-LU-1, and NCI–H1299 cells were cultured in DMEM medium (GIBCO, Rockville, MD, USA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA) and penicillin (100 U/mL)/ streptomycin (100 μg/mL) (Hyclone). NCI–H292, NCI–H358, and NCI–H1975 cells were cultured with 10% FBS supplemented with penicillin/streptomycin in RPMI 1640 medium (GIBCO). Cells were maintained at 37 °C in a humidified 5% CO₂ incubator. MG132 (S2619), cycloheximide (S7418), Piperlongumine (S7551), Puromycin (S7417), or WP1130 (S2243) were purchased from Selleck Chemicals (Houston, USA). Blasticidin (203351), polybrene (TR-1003), and anti-FLAG® M2 affinity gel (A2220) were purchased from Sigma-Aldrich (St. Louis, USA). Pierce™ Anti-HA magnetic beads (88836) were purchased from Thermo Fisher Scientific. Diphenylterazine (HY-111382) was purchased from MCE (Shanghai, China). N-acetylcysteine (ST1546) was purchased from Beyotime (Shanghai, China).