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4 protocols using peg10k

1

PEG10k-Citrate Buffer Solution Preparation

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20% (w/v) PEG10k (Mw = 10 kDa, SigmaAldrich, St. Louis, MO) aqueous solution (pH = 3 and 8) was prepared by dissolving 20 g of PEG10k in distilled water, where the final desired pH was adjusted using hydrochloric acid (HCl) or sodium hydroxide (NaOH) solution. 19.54 g of citric acid monohydrate (Mw = 210.14 g mol−1, SigmaAldrich, St. Louis, MO) and 2.06 g of sodium citrate tribasic dihydrate (Mw = 294.12 g mol−1, SigmaAldrich, St. Louis, MO) were dissolved in 80 mL of distilled water and then the final solution pH was adjusted to 3, followed by adding distilled water until the final volume was 100 mL to prepare 1 M sodium citrate buffer solution at pH = 3. Then, 20 g of PEG10k was dissolved in this buffer solution.
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2

Preparation of PEG10k Solutions

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20% (w/v) PEG10k (M w = 10 kDa, SigmaAldrich, St. Louis, MO) aqueous solution (pH = 3 and 8) was prepared by dissolving 20 g of PEG10k in distilled water, where the final desired pH was adjusted using hydrochloric acid (HCl) or sodium hydroxide (NaOH) solution. 19.54 g of citric acid monohydrate (M w = 210.14 g mol -1 , SigmaAldrich, St. Louis, MO) and 2.06 g of sodium citrate tribasic dihydrate (M w = 294.12 g mol -1 , SigmaAldrich, St. Louis, MO) were dissolved in 80 mL of distilled water and then the final solution pH was adjusted to 3, followed by adding distilled water until the final volume was 100 mL to prepare 1 M sodium citrate buffer solution at pH = 3. Then, 20 g of PEG10k was dissolved in this buffer solution.
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3

Synthesis and Characterization of Nanomedicines

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Tannic acid, PEG10k, F-68, KO2, α-amylase, xanthine oxidase, BSA, fluorescein 5(6)-isothiocyanate, pepsin (from porcine gastric mucosa) and pancreatin (from porcine pancreas) were bought from Sigma-Aldrich. DSPE-PEG2k was obtained from Advanced Vehicle Technology Pharmaceutical Co., Ltd (Shanghai, China). Cy5.5-NHS were purchased from (ApexBio Technology, USA). Cytochrome C was obtained from Sangon Biotech (Shanghai, China). Infliximab was purchased from BioChemPartner (Shanghai, China). Dextran sodium sulfate (molecular weight: 36,000-50,000 Da) were purchased from MP Biomedicals Inc. (USA).
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4

Synthesis of Octabutoxy Naphthalocyanine

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5,9,14,18,23,27,32,36-Octabutoxy-2,3-naphthalocyanine (ONc) was from HPPE Co., tin dichloride was from Alfa Aesar, PEG-10K, PEG 20-K and silver hexafluorophosphate were from Sigma Aldrich, TWEEN 20 was from Amresco, Biobeads SX-1 were from Bio-Rad Laboratories, XTT was from Biotium. ICR mice were ordered from Harlan Laboratories.
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