Cells isolated from the spleen, liver, and intestinal epithelium were stained with LIVE/DEAD Fixable Aqua Dead Cell Stain kit (L3457; Thermo Fisher, USA) and the following fluorescent antibodies to chicken CD3 (CT-3, Southern Biotech, USA), CD4 (CT-4, Southern Biotech, USA), CD8α (CT-8, Southern Biotech, USA), CD44 (AV6, Southern Biotech, USA), TCRγδ (TCR-1, Southern Biotech, USA), monocytes/macrophages (KUL01, Southern Biotech, USA), MHCII (2G11, Southern Biotech, USA), Bu-1 (AV20, Southern Biotech, USA), and CD45 (LT-40, Southern Biotech, USA). Stained cells were analyzed using a CytoFLEX flow cytometry and the data were analyzed using CytExpert Software (both from Beckman Coulter, USA).
Mhcii 2g11
Manufactured by Southern Biotech
Sourced in United States
MHCII (2G11) is a monoclonal antibody that binds to the major histocompatibility complex class II (MHC II) protein. MHC II proteins are cell surface receptors expressed on certain immune cells, such as antigen-presenting cells, and play a crucial role in the adaptive immune response. The MHCII (2G11) antibody can be used in various immunological and cell biology applications for the detection and analysis of MHC II-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Bu 1 av20, by Southern Biotech (2 mentions)
Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Cytoflex flow cytometry, by Beckman Coulter (1 mentions)
Kul01, by Southern Biotech (1 mentions)
Cytexpert software, by Beckman Coulter (1 mentions)
Cd8α ct 8, by Southern Biotech (1 mentions)
Cyto ex, by Beckman Coulter (1 mentions)
Live dead fixable aqua dead cell stain, by Thermo Fisher Scientific (1 mentions)
2 protocols using mhcii 2g11
1
Multiparametric Immune Profiling of Avian Tissues
2
Multiparameter Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cultured splenocytes were harvested and stained with the LIVE/DEAD Fixable Aqua Dead Cell Stain (L3457; Thermo Fisher) and antibodies speci c for CD4 (CT-4, SouthernBiotech), CD8α (CT-8, SouthernBiotech), CD44 (AV6, SouthernBiotech), Monocyte/Macrophage (KUK01, SouthernBiotech), MHCII (2G11, SouthernBiotech), Bu-1 (AV20, SouthernBiotech). Stained cells were analyzed using ow cytometry (Cyto ex, Beckman Coulter).
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