Eos 600d

Planarian live and WISH images were acquired with Canon EOS 600D and OLYMPUS SZX10 microscopes, respectively. Z-stacks of fluorescently labeled samples were acquired with a LEICA SP8 confocal microscope and processed with the ImageJ package Fiji. For quantification of H3P-positive or TUNEL-positive cells, stacks with equal numbers of z-sections were taken and cells were counted in the indicated areas using ImageJ package Fiji. To quantify cilia density, the fluorescence intensity of the Ac-tubulin signal was measured and normalized to DAPI fluorescence. To assess blastema formation, the length of the regenerated blastema was measured using ImageJ package Fiji and normalized to the whole-body length of the trunk. The ratio of blastema/whole-body length of small molecules treated animals or Djpi3k(RNAi) animals were compared with control animals at each time point.

Pinned specimens were photographed with an Olympus E-410 digital camera attached to an Olympus SZX12 microscope or with Canon 750D and MP-E-65 mm lens. Slide-mounted genitalia were photographed with a Canon EOS 600D digital camera mounted on an Olympus U-CTR30-2 trinocular head combined with a Carl Zeiss microscope body. Sets of 10–20 images were taken for each specimen and assembled to deep-focused images using Helicon Focus 6 and edited in Adobe Photoshop CS5.

The deflection as a function of voltage are obtained with the colorimetry technique with an Olympus BX60 microscope as previously reported^{6 (link),7 (link)}. Pictures are taken with a Canon EOS 600D and a 20× Olympus UMPlanFI objective lens (NA 0.46, LWD 3 mm). A TE-cooled linear CCD array spectrometer (B&W Tek Glacier X) is attached to the optical microscope in such a way that it collects the light reflected from an area slightly larger than the selected drum (about 595 μm²) (Supplementary Note 1 and Supplementary Figure 1). Spectral traces of the entire drum are recorded with an integration time of 20 ms (no averaging). For the electrostatic pulling, we use a power supply (Rigol DP832) to ramp up the voltage from 0 to 20 V with steps of 0.25 V. The voltage is left constant for a period of 30 s after each step and before acquiring the data.