Dulbecco s modified eagle s medium high glucose dmem hg

2,5-Furandicarboxylic acid (>98%) was purchased from TCI Europe NV (Zwijndrecht, Belgium). 2-Ethyl-1-hexanol (≥99.6%), stearic acid (95%), anhydrous sodium sulfate (≥99.0%), zinc stearate purum, and Dulbecco’s modified Eagle’s medium—high glucose (DMEM-HG) were supplied by Sigma Aldrich-chemicals Corp. (Sintra, Portugal). Sulfuric acid (96%), sodium chloride (≥99.0%), and deuterated Chloroform (99.8% D) were acquired from Acros Organics (Geel, Belgium). Chloroform and cyclohexane (HPLC grade) were purchased from Fisher Scientific (Porto Salvo, Portugal and Panreac Applichem (Barcelona, Spain), respectively. Activated carbon (granules with density of 2 g cm⁻³) was obtained from VWR Chemicals. Di(2-ethyl-1-hexyl) 1,4-terephthalate (DEHT) (DOTP-168 Eastman) was supplied by Eastman Chemical Company (Madrid, Spain) and PVC resin (VICIR S1200; The K-Fikentscher value of 70, corresponding to number-average molecular weight equal to 59000 and a polydispersity of 1.97) [22 (link)], was provided from CIRES, Lda. (Portugal). All chemicals were used as received, without further purification.

Anhydrous sodium carbonate, Folin–Ciocalteu’s phenol reagent, ethyl acetate (HPLC gradient grade), methanol (analytical reagent and HPLC gradient grade), 1-butanol, chloroform were purchased from Merck (Darmstadt, Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), gallic acid, 2,6-di-tert-butyl-4-methylphenol (BHT), phenolic standards, formic acid, fetal bovine serum (FBS), penicillin/streptomycin, dimethyl sulphoxide (DMSO), Dulbecco’s modified Eagle’s medium-high glucose (DMEM-HG), gallic acid, 4-hydroxybenzoic acid, apigenin-7-glucoside, and 5-fluorouracil were purchased from Sigma-Aldrich GmbH. (Taufkirchen, Germany). All solvents used in HPLC analysis were HPLC grade and purchased from Merck.

NMR spectra were recorded on a spectrometer operated at 400 MHz for ¹H and 100 MHz for ¹³C NMR. Chemical shifts were given in ppm (δ scale), coupling constant (J) in Hz. Column chromatography was performed on silica (60–230 mesh, Merck). TLC was carried out on analytical alumina plates (60 F₂₅₄). Hitachi U-290 UV-Vis spectrometer was used for UV measurements. HRMS analyses were recorded on Agilent 6210 LC-TOF/MS spectrometer. Ammonium thiocyanate and BHT were purchased from E. Merck (Darmstadt, Germany). Ferrous chloride, BHA and trichloracetic acid (TCA), formic acid, foetal bovine serum (FBS), penicillin/streptomycin and Dulbecco’s modified Eagle’s medium-high glucose (DMEM-HG) were purchased from Sigma-Aldrich (Darmstadt, Germany).