Bs1098

Immunofluorescence assays were used to detect the expression of E-cadherin in GES-1 cells cultured with the CM of macrophage-hucMSCs (28 (link)). GES-1 cells cultured with the CM of macrophage-hucMSCs in 24-well plates for 48 h were washed three times with PBS, fixed with 4% paraformaldehyde at room temperature for 20 min, permeabilized with 0.1% Triton X-100 for 5 min, blocked with 5% bovine serum albumin (Boster Biological Technology, Pleasanton, CA, USA) at room temperature for 30 min, and then incubated with anti-E-cadherin antibodies at a dilution of 1:100 (cat. no. BS1098; Bioworld Technology, Inc.) at 4°C overnight, followed by incubation with a fluorescein isothiocyanate (FITC)-conjugated anti-rabbit secondary antibody at dilution of 1:200 (F9887; Sigma-Aldrich; Merck KGaA) at 37°C for 1 h. The cells were then stained with DAPI (Beyotime Institute of Biotechnology) for nuclear staining. Images were then acquired with a Nikon TE300 Inverted Fluorescence Phase Contrast Microscope (Nikon Corporation, Tokyo, Japan), magnification, ×200.