Dylight 594 conjugated secondary antibody a23410

HEK-293, C3H10T1/2, and C2C12 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). The above cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM), which contained 10% (v/v) fetal bovine serum (FBS), penicillin (100 U/mL), and streptomycin (100 μg/mL). Cell culturing dishes, flasks, 6-well plates, and 24-well plates were incubated at 37 °C with 5% CO₂. DMEM, FBS, and trypsin were purchased from Gibco (Chinese branch). BMP9 (sc-514211), ALP (sc-271431), RUNX2 (sc-12488), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, sc-32233) antibodies, retinoic acid receptor beta (RARβ) specific inhibitor Le135 (sc-204053), and ATRA (sc-200898) were bought from Santa Cruz Biotechnology (Chinese branch). Osteopontin (OPN, ab8448) and RARβ (ab53161) antibodies were purchased from Abcam (Chinese branch). Serpina3n (AF4709) antibody was sourced from R&D systems (Chinese branch). Dexamethasone (D4902) was obtained from Sigma-Aldrich (Chinese branch). Dylight 594 conjugated secondary antibody (A23410) was purchased from Abbkine (Chinese branch). Alkaline Phosphatase Assay Kit (C3206) and Alizarin Red S (ST1078) were bought from Beyotime (Shanghai, China).

C3H10T1/2, C2C12, and HEK-293 cell lines were purchased from ATCC (Manassas, VA, USA). Cells were cultured in dishes, flasks, or plates with Dulbecco's modified Eagle's medium which contained 10% fetal bovine serum (FBS), streptomycin (100 μg/mL), and penicillin (100 U/mL). These dishes, flasks, and plates were cultured in 37°C incubators with 5% CO₂. Primary antibodies against Runx2 (sc-390715), OPN (sc-21742), β-catenin (sc-7963), GSK-3β (sc-81462), p-GSK-3β (sc-81496), and PDK4 (sc-518103) were bought from Santa Cruz Biotechnology (CA, USA); GAPDH (10494-1-AP) was purchased from Proteintech (Wuhan, China); COXIV was ordered from Cell Signaling Technology (Boston, USA). Dylight 594 conjugated secondary antibody (A23410) was bought from Abbkine (Chinese branch). Alizarin Red S (ST1078) and Alkaline Phosphatase Assay Kit (C3206) were obtained from Beyotime (Shanghai, China).