Btg2 sirna

Manufactured by GenePharma

Sourced in China

Btg2 siRNA is a laboratory reagent used for gene expression studies. It is a short interfering RNA (siRNA) molecule designed to target and suppress the expression of the Btg2 gene. The Btg2 gene is involved in various cellular processes and its downregulation can be used to investigate the gene's functions. This product is intended for research use only and its performance and suitability for specific applications should be verified by the user.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Gp transfect mate transfection reagent, by GenePharma (1 mentions) P53 sirna, by GenePharma (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Si nc, by GenePharma (1 mentions)

2 protocols using btg2 sirna

siRNA-mediated Gene Knockdown and Overexpression

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Cells were seeded in 6-well plates. After 24 h, 75 nM siRNA was transfected using GP-transfect-Mate transfection reagent (GenePharma, China) according to the manufacturer’s instructions. Cells were harvested two days following the initial transfection. Knockdown was validated prior to all experiments reported in this study by immunoblot analysis. Negative control siRNA from GenePharma was used as control siRNA in knockdown experiments. Ythdf3 siRNA, Cbln1 siRNA, and Btg2 siRNA were also ordered from GenePharma. Table S2 shows the siRNA sequences used in gene knockdown experiments. In addition, Ythdf3 CDS was synthesized by Sangon Biotech Company (Sangon Biotech Co., Ltd., Shanghai, China) and inserted into pcDNA3.1(+) Vector using the HindIII or XhoI restriction sites. 1.5 μg plasmid were transfected into N2a cells using lipofectamine 2000 (Invitrogen) in overexpression experiments. Overexpression was also validated prior to all experiments reported in this study by immunoblot analysis.

Lu L., Shi Y., Wei B., Li W., Yu X., Zhao Y., Yu D, & Sun M. (2023). YTHDF3 modulates the Cbln1 level by recruiting BTG2 and is implicated in the impaired cognition of prenatal hypoxia offspring. iScience, 27(1), 108703.

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Targeted Silencing of BTG2 and p53

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BTG2 siRNA, p53 siRNA, and siNC were designed and synthesized by GenePharma (China).
Cell transfection was performed using Lipofectamine 3000 (Invitrogen Life
Technologies) according to the manufacturer's instructions.

Zhao L.M, & Pang A.X. (2017). Iodine-131 treatment of thyroid cancer cells leads to suppression of cell proliferation followed by induction of cell apoptosis and cell cycle arrest by regulation of B-cell translocation gene 2-mediated JNK/NF-κB pathways. Brazilian Journal of Medical and Biological Research, 50(1), e5933.

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