Wako l type tg m kit

All animals used in the study were in C57BL/6 background. Our study used male mice unless otherwise specified. Mice were fed chow diet (Research Diet) and housed temperature-controlled room under a 12-h light/12-h dark cycle and pathogen-free conditions. Raly^flox/flox mice were generated by Cyagen using the strategy outlined in Fig. 1a. To the generated RALY liver-specific knockout mice and littermate controls, we treated Raly^flox/flox with adeno-associated virus (AAV) with TBG promoter (AAV8.TBG.Cre) or (AAV8.TBG.GFP; green fluorescent protein) purchased from Penn Vector Core. AAV administered intraperitoneal injection at dose of 5 × 10¹¹ GC per mice. Mice were euthanized 4 weeks after AAV injection. Liver tissues were frozen in liquid nitrogen and stored at −80 °C or fixed in 10% formalin. Blood was collected by retro-orbital bleeding, and the plasma was separated by centrifugation. Plasma lipids were measured with the Wako L-Type TG M kit, the Wako Cholesterol E kit. All animal experiments were approved by the UCLA Institutional Animal Care and Research Advisory Committee.